Evaluation Of Laboratory Tests In Diagnosis Of Thrombotic Diseases

Objective: To evaluate the biomarkers of endothelium, coagulation, fibrinolysis and platelet in diagnosis of thrombotic diseases.Materials and methods: 1. Fifty-one patients with thrombotic diseases as thrombotic group, twenty-one patients without thrombotic diseases as non-thrombotic group, and 35 healthy people as control group, were prospectively investigated at Huaxi Hospital from Oct of 2002 to Jan of 2003. The levels of plasma von Willebrand factor antigen (vWF:Ag), tissue factor (TF), prothrombin fragment 1+2 (F1+2), tissue-plasminogen activator antigen (t-PA:Ag), thromboxane B₂ (TXB₂) were detected by ELISA, The levels of plasminogen activator inhibitor activity (PAI:Ac) and α₂-plasmin inhibitor activity (α₂-PI:Ac) were detected by chromogenic assays. The results were analyzed by ANOVA, Non-parametric test or Student t-test in order to find out whether there are differences in these groups. Receiver-operator curve (ROC) analysis and calculated indices such as sensitivity (Sen) and specificity (Spe), positive predictive value (PPV) and negative predictive value (NPV), positive likelihood ratio (pLR) and thereciprocal of negative likelihood ratio (1/nLR), odds ratio (OR) and area under ROC curve (AUC) were used to evaluate and compare the diagnostic value of the results.Results: The levels of t-PA:Ag, PALAc, a2-PI:Ac, vWF:Ag and Fl+2 were significantly higher in thrombotic or non-thrombotic group than those in control groups < 0.05). However, there were no significant differences in ot2-PI:Ac, vWF:Ag, Fl+2 values between the thrombotic and non-thrombotic group (p > 0.05). The results of Sen, Spe, OR, and AUC for t-PA:Ag in thrombotic group were 66.7%, 66.7%, 4.0, and 0.690, respectively, which were compared with 94.1%, 60.7%, 24.7, and 0.849 for PALAc, respectively.Conclusion: Our results suggest that PALAc and t-PA:Ag could be sensitive as the screening tests in diagnosis of thombotic diseases that were mostly arterial thrombosis; vWF:Ag,