Clinical Application Of Circulating Autoantibodies In Diagnosis And Differential Diagnosis Of Benign And Malignant Pulmonary Diseases

Backgroud Lung cancer is one of the most common cancers in the world,ranking first in male cancer mortality and second in female cancer mortality after breast cancer.Lung cancer has the highest morbidity and mortality in high-income countries such as Europe,In North America,the earliest countries that started smoking have seen a marked decrease in their incidence and mortality in recent years,while the prevalence of lung cancer in some low-income and middle-income countries continues to rise as a series of problems such as environmental pollution,changes in lifestyles and population aging.The diagnosis of lung cancer,from cytology and histopathological examination to detect serum tumor markers,and then combined detection of tumor markers,sensitivity and specificity are improved.However,the early diagnosis of lung cancer,autoantibodies sensitivity and specificity showed greater superiority.Objective: To evaluate the diagnostic value of seven autoantibodies in the differential diagnosis of lung cancer patients.Methods: The serum levels of seven autoantibodies P53,PGP9.5,SOX2,GAGE7,GBU4-5,MAGEA1 and CAGE were detected by ELISA in 122 subjects,which include 65 extrapulmonary tumors,20 benign lung diseases,3 small cell lung cancers,20 physical examination subjects and 14 non-small cell lung cancers.Subsequently,the results were statistically analyzed by SPSS.Results: The subjects were divided into five subgroups,included extrapulmonary tumors,benign pulmonary lesions,small cell lung cancer,physical examination and non-small cell lung cancer subgroup.P53,SOX2,GBU4-5 and MAGEA1 in small cell cancer subgroup had significantly higher expression levels.Subjects were categorized differently using a ROC curve for statistical analysis.The results showed that P53(AUC = 0.664,P<0.05)was significantly higher in lung cancer group than another group.GBU4-5(AUC = 0.639,P <0.05)and the combination of the seven indicators(AUC = 0.635,P <0.05)had a certain diagnostic value for differential diagnosis of the non-malignant tumor group and the tumor group.MAGEA1,CAGE,PGP9.5,SOX2 and the combination of the seven indexes(AUC> 0.8,P <0.05)had a high value in the differential diagnosis of small cell lung cancer group and another group.MAGEA1(AUC = 0.333,P <0.05)had some value in the differential diagnosis of the physical examination group and another group.P53(AUC = 0.33),PGP9.5(AUC = 0.355),GBU4-5(AUC = 0.280),CAGE(AUC =0.341)and seven indicators combined(AUC = 0.731)had certain value in the differential diagnosis of benign lung diseases group and another group.Conclusions: According to the different groups,the detection of seven autoantibodies and their combi ned had a certain value for differential diagnosis of lung cancer.