Association Of HLA-DRB1 With Vitiligo In Hans Of Ji Lin Region

Vitiligo is generally recognized as a dermatogic diseasecharacterized by destruction of melanocyte in small or largecircumscribed areas of skin, resulting in patches of depigmentation.Histologically. Skin shows losses of functional melanocytes andmelanin within the epidermis. It can occur in any body parts andany ages. It occurs in world about 0.5% of the population and asfrequently males as it does in females.The exact pathogenisis of vitiligo is unclear, but might involveautoimmunity, heredity, neurogenic cause, noxius product ofmetabolism and lack of melanocyte growth factors. Thought thereweren't extinct reports that vitiligo has clear complication withheredit susceptibility, family habitation and linkage disequilibriumof genes showed that herdit factors played an important role in theaccurance of vitiligo. And vitiligo was proved to be multi-geneinherent. Current research showed that vitiligo related with geneson different chromosomes, especially human leucocyte antigen(HLA). HLA located in 6P21.31, whole length (3.5～4)×103kb andabout the length of 1/3000 of human genome, included 224 geneseats. Among them, 128 functional genes and 96 pseudogenes.Many genes included a few multiple allele, had a fewpolymorphism. According to the constructions, histologicalconstitution and functions of gene products, HLA was divided intoHLA -Ⅰ,II and Ⅲ.Tape II genes was 1000kb, had threesubregions (DR, DQ, DP). HLA-II molecules located in antigenpresenting cells (APC), B cells and activated T cells, and canpresent antigens to T cell receptor (TCR) , take part in the selectivedevelopment of TCR. In the past, more researches were about HLA -Ⅰ, and thenhuman found that HLA -Ⅱplayed an more important role inaccordance with some autoimmune diseases. Especially HLA-DRgene. Researches from inside or outside showes that certainHLA-DR genes association may be risky factors for vitiligo. Thereare some views that immunoresponsive genes which regulatingimmue eactions were included by HLA-DR genes and specificgenetypes of HLA-DR genes induce abnormal immune responses.On this background, DR antigens form the new antigenicity duringpresenting foreign antigens to T cells, and it can make the cellswith abnormal antigens not be soluted by NK cells or LAK cells,escaping immunological surveillance, inducing affectability tovitiligo. It has significant value that the association between DRgenes polymorphism and vitiligo is investigated for exploringetiopathogenisis and pathogenesis of vitiligo. HLA systems aredifferent because of the diversity of races, nations and environment.So we selected vitiligo patients in Ji Lin region as objects andexplored genetic immunity pathogenesis of vitiligo from gene levelfurther by detecting the distribution of HLA-DRB1 betweenhealthy persons and patients. In our experiment, PCR-SSP method was used to test, and itsprinciple was that design specialized primers according to someknown nucleotide sequences. Amplify the specialized fragments toanalyze the polymorphism of HLA-DR genes. This method hadhighly specificity and resolution, and can operate faster and easier.It can repeat and judge the results easily, was a good way inanalyzing gene types. In our experiment, 82 vitiligo patients and 86 healthy personswere studied for HLA-DRB1 allelic polymorphism by polymerasechain reaction sequence-specific primer method (PCR-SSP). Thecomparison of the patients and the control group showed asignificant increase in the frequency of the HLA-DRB1*07 andHLA-DRB1*12 alleles in the patient group (P<0.01). So HLA-DRB1*07and HLA-DRB1*12 alleles might contribute to geneticsusceptibility to vitiligo of Ji Lin region. Our research is different from reports outside or in; the reasonis that HLA systems are difference because of the diversity of race,nation and environment. HLA gene is not the real pathogenicreason; just occupying close linkage disequilibrium with the...