Association Of Polymorphisms Of TNF-α-238G/A, HLA-DRB1 And HLA-DQA1 Gene With Outcomes Of Hepatitis B Virus Infection In Han Population Of North China

The clinical outcomes of HBV infection are extremely variable, and might be associated with the genetic factors. Immune reaction initiated by the T-cell response to viral antigens is supposed to play a fundamental and important role for viral clearance and disease pathogenesis in HBV infection. Human leucocytes antigen (HLA) may be associated with the control of immune reaction. Tumor necrosis factor-α (TNF-α) may play an important role in HBV elimination and the immune reaction of host responding to HBV. A case-control study was used to analyze the relationship between gene polymorphisms and outcomes of HBV infection, the gene-environment interaction and its effects on the outcomes of HBV infection. This study included 207 chronic HB patients, 212 chronic asymptomatic HBV carriers (HBV carrier) and 148 self-limited HBV infection individuals. The polymorphisms of TNF-α -238, HLA-DRB1 and HLA-DQA1 gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-sequence special primers (PCR-SSP) in genomic DNA. The results of the study are as follows:1. The frequency of TNF-α-238GG genotype was 98.07% in chronic HB patients, 91.98% in HBV carriers and 93.24% in self-limited HBV infection subjects, respectively. The frequency of the G allele in patients with chronic HB was significantly higher than that in subjects with HBV carriers (x~2=7.94, P=0.0048) and self-limited HBV infection subjects (x~2=5.20, P=0.0226). There was no significant difference of G allele frequency between HBV carriers and self-limited HBV infection subjects (x~2=0.19, P=0.6610).2. The frequency of HLA-DRB1*01 in self-limited HBV infection subjects (5.41%) was higher than in HBV carriers (2.36%) (x~2=4.65, P=0.0311), and the frequency of HLA-DRB1*12 in chronic HB patients (17.32%) was higher than that in HBV carriers (12.03%) (x~2=4.67, P=0.0307). The frequency of HLA-DRB1*09 in HBV carriers (20.52%) was much higher than that in chronic HB patients (13.66%) (x~2=6.91,...