Effects Of Static Magnetic Field On The Activity Of Alkaline Phosphatase And Superoxide Dismutase Of Human Gingival Fibroblasts

The application of oral magnets has been broaden increasingly in recent years, especially for the magnetic attachments. As a new type of sources for denture retentive force, the magnetic attachments have shown their unique advantages in the domain of prosthetic dentistry. In their course of development from early open-circuit magnet system to nowadays close-circuit magnet system, for different type of magnets, controversial opinions persist about whether their static magnetic fields have oral biosecurity. Among those opinions, more emphasis has been paid to the effects of magnets on the periodontal tissues.In this study, a magnet loading system which was developed by the department of prosthetic dentistry in the West Chine college of stomatology, Sichuan university, was used to impose the static magnetic force on the human gingival fibroblasts (HGF) in vitro to investigate if there was any activity change of alkaline phosphatase (ALP) and superoxide dismutase (SOD) in those cells. The 120mTand lOmT magnetic fields generated by the system were designed to stimulate the effects of open-circuit and close-circuit magnetic attachments on the gingiva respectively. For each amount of magnetic fields intensity, 3 groups of repeated loading process with intervals were carried out with different numbers of 24-hour periodic cycles. And automatic biochemical analyzer and a SOD test box were applied to measure the activity of ALP and SOD accordingly. Comparison between the test and control groups that differ in their magnetic intensity and loading time was also made to analyze the biological effects of the static magnetic fields. The results show as following:1. No significant difference (P>0.05) of ALP or SOD activity was found between all the groups when HGF were loaded with different intensity of magnetic fields for the same period of time;2. Nor any significant difference (P>0.05 ) of ALP or SOD activity was found between all the groups when HGF were loaded with the same intensity of magnetic fields for different periods of time.As far as the conditions in this study are concerned, the results above supported that with reference to the ALP and SOD in HGF, biosecurity cloud be announced for the static magnetic fields of the open-circuit magnetic attachments as well as the close-circuit ones.