Screen TPC And Study It's General Pharmacolog Effects

Objective:To screen TPC and study it's general pharmacolog effects. Methods:Orthogonal design was used to prepare TPC with TP ,Egb761 , PNS ,DS. Themaximal-tolerant-dosage method was used to observe the acute-toxicity of TPC.Anoxic tolerance was measured by antihypoxia method .The initial stability ofTPC was measured by HPLC . Results: The best compound of TPC was screened.TPC was low toxicity and all mice were alive with i.g. 7 g.kg-1.d-1 of TPC for 7d .The anoxic tolerance of mice increased significantly with dosage dependence byi.g. 175,350,700 mg.kg-1.d-1 of TPC respectively. Among the total,the high dosagewas best.Comparing with the vacuo , the survival time of mice elongated 48.93%.TheEGCG's content of TPC was measured at regular intervals for five times within 2hby HPLC , and the RSD was 1.98% .Conclusions: The best compound of TPC isscreened in preliminary experiment.TPC is low toxicity. TPC can increase theanoxic tolerance of mice. The TPC solution is stable within 2h at roomtemperature.