| Background and aimThromboembolistic diseases are threatening human's lives. Large numbers of research in foundation and clinical makes know that multielement brings thrombosis on synthetically, which are vascular endothelial, blood platelets coagulation, fibrinolysis, hemorheology, and so on. Vascular endothelial injury weakens the function of anti-thrombosis and hemostasis, blood begin to coagulate, Hence thrombosis startups abnormally, the prethrombotic state comes into being. Thereforce, People pay more attention to the function of vascular endothelial to prevent and cure the occurrence and expandsion of Thromboemblism.The traditional Chinese Medicine think Qi and blood are affinitative each other, they depend on in physiology, "Qi captain blood"; and affect inpathology, Qi deficient could not act the circulation of blood, this make the blood flow slowly, blood stasis is formed at last. Blood stasis in essence has approved to be the activation of blood platelet, the unbalance of coagulation and flbrinolysis, the change of hemorheology and microcirculation of blood, and so on. At the present time, research has evidenced blood stasis is in connection with vascular endothelial injury, and the herbal medicine of activating blood circulation has an effect on it.So, In our study, we plan to observe the effects of Qidantongmai tablet (QDTMT), a compound preparation made up of pure traditional Chinese herbs, on anti-thrombosis function of endothelial cells , the prethrombotic state and the expression of vWF and E-selectin on mice with vascular endothelial injury, aiming at further research on activating blood circulation to remove blood stasis, supplying reliable experimental basis for the clinic application of this medicine to prevent and cure ischemic heart disease , ischemic cerebrovascular disease , vascular disease of diabetes mellitus, et al.Methods1. The establish of animal modelSixty healthy male SD mice were divided randomly into six groups, ten mice per group: control group(Control), model group(Model), QDTMT high dosage(QDTMTH), middle dosage(QDTMT_M), low dosage(QDTMT_L) and herbesser groups, Then Saline was given to control and model at 10 ml.kg-1 weight; Suspension of QDTMT were give to QDTMTH, QDTMTM, QDTMTLat dose of 3.24 g·kg-1·d-1, 1.08 g·kg-1·d-1, 0.36 g·kg-1 weight; HB at dose of 5 mg·kg-1 weight was given to herbesser group for 2 weeks. After 14-day administration of QDTMT, the model of vascular endothelial injurywas established by injecting a high close of adrenaline solution to the SD mice subcutaneously for 5 day.2. Tissue sample collection and treatmentPut mice into narcotism and blood from heart; In time, the blood with anticoagulant was centrifugal, and then the blood plasma preserved at -20°C; The aorta descendens keep to immunohistochemistry sample.3. ELISA method and immunohistochemistry were use to detect the expression of E-selectin,The activity of AT-III n vWF and the concentration of D-D were determined automatically, The concentration of 6-Keto-PGFia and TXB2 were determined by immunoradiometric assay, The expressed protein of vWF were detected by Western-Blot.Results1. No expression of E-selectin was found in the control group, but the expression of E-selectin was found in Adrenaline (Adrenaline,Adr) groups, positive expression decreased significantly in the group of QDTMTh ?2. Compared to model group, the activity of AT-III and the concentration of 6-Keto-PGFia and odd of 6-Keto-PGFia/TXB2 were increased; The activity of vWF was lowered singnificantly in the group of QDTMTh and QDTMTM, the effects of QDTMTH was obviously better than those of QDTMTM and Herbesser3. Compared to model group, the concentration of D-D and TXB2were decreased, the activity of AT-III and the concentration of 6-Keto-PGFia were increased very singnificantly in the group of QDTMTh and QDTMTm, the effects of QDTMTh was obviously better than those of QDTMTm and Herbesser.4. Compared to model group, the concentration of E-selectin was increased very singnificantly in the group of QDTMTh and QDTMTm, the... |
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