| Objective Succeed in duplicating little old and feeble mouse's model and regard hiding the starting point like the heart characteristic as, Yang Zang-organ for the heart, mainly warm mainly open, main fact of blood, cases of respect of pulse, use the treatment of reinforcing Qi and promoting blood circulation to remove blood stasis, probe into heart hide like the relation with aging, announce tentatively that stores it in the scientific intension of the anti-aging respect like the heart.Methods 1.This experiment sets up 6 groups: Plant weight 20±2 grams of little" mouse in 72, 3 months old Kunming, male and female half and half, divide cage into to be ordinary to raise 6 day so as to environment of surrounding, divided into 6 groups at random after observe and has not unusually reactedding, among them 12 are regarded as the normal group, other 60 model group, reinforcing Qi group, promoting blood circulation to remove blood stasis group, reinforcing Qi and promoting blood circulation to remove blood stasis group and Vitamin E group respectively. Adopt each little mouse every day neck dorsal, 1g of hypodermic injection, to model group and medicine group subsequently; 1%D -Galactose 0.1ml cause subnormal temperature old and feeble model, normal group little mouse every day neck dorsal hypodermic injection physiological saline 0.1ml, inject for 6 weeks continuously. While injecting D - Galactose from the 7th day, every medicine group begins to give medicine, adopt and irritate the stomach method and give medicine, the reinforcing Qi group presses of 5g(kg·d)-1 offer reinforcing Qi medicine to fry unguentum mix liquid (0.5g/ml); the promoting blood circulation to remove blood stasis group presses of 5g (kg·d)-1 offer promoting blood circulation to remove blood stasis medicine to fry unguentum mix liquid (0.5g/ml); The reinforcing Qi and promoting blood circulation to remove blood stasis group of benefit presses of 7.5 g(kg·d)-1 is offered reinforcing Qi and promoting blood circulation to remove blood stasis medicine to fry unguentum to mix the liquid (0.75g/ml); The Western medicine group presses of 50 mg (kg·d)-1 mixes, hangs the liquid (5mg/ml) the Vitamin E; Once a day, for five weeks in succession. Normal group and model group offer, etc. the physiological saline of capacity irritates the stomach, Once a day, for five weeks in succession. Little mouse's free diet of experiments. 2.Medicine: Reinforcing Qi medicine is made up of sun-dried ginseng 15g, Chinese angelica 10g and cassia twig 10g, concentrate this side as traditional Chinese medicine preparation containing crude medicine 0.5g/ml, people mouse weight it lasts amounts of medicine given of mouse little to be 0.2ml / only/day; Promoting blood circulation to remove blood stasis medicine is made up of leech 10g, ground beetle's worm 5g, earthworm 10g and cassia twig 10g, concentrate this side as traditional Chinese medicine preparation containing crude medicine 0.5g/ml, it it lasts amounts of medicine given of mouse little to be 0.2ml according to people mouse weight / only/day; Reinforcing Qi and promoting blood circulation to remove blood stasis medicine is made up of sun-dried ginseng 15g, Chinese angelica 10g, leech 10g, ground beetle's worm 5g, earthworm 10g and cassia twig 10g, this it concentrate side it last amount of medicine given 0.2ml of mouses little to traditional Chinese medicine preparations containing crude medicine 0.75g/ml according to people mouse weight/only/day; Worthy of become mix liquid will be hung Vitamin E glue pill, last amount of medicine given 0.2ml of mouses little according to people mouse weight 0.2ml / only/day; Normal group, model group offer, etc. the physiological saline of capacity irritates the stomach, the number of times and course of treatment are the same as medicine groups. 3.Then measure angry medicine of benefit separately, reinforcing Qi medicine, promoting blood circulation to remove blood stasis medicine, reinforcing Qi and promoting blood circulation to remove blood stasis medicine and Vitamin E to old and feeble model little mouse auricle little circulation, acetylcholinesterase, myocardium cell ultra influence of micro-structure.Results 1. Reinforcing Qi medicine, promoting blood circulation to remove blood stasis medicine, reinforcing Qi and promoting blood circulation to remove blood stasis medicine obviously expand function to old and feeble model little mouse auricle arteriole, little vein, make blood vessel blood pace increase obviously, reinforcing Qi and promoting blood circulation to remove blood stasis medicine group result superior to promoting blood circulation to remove blood stasis group, reinforcing Qi and promoting blood circulation to remove blood stasis group and Vitamin E group obviously among them. Reveal it has good little circulation function of improvement; 2. Reinforcing Qi medicine, promoting blood circulation to remove blood stasis medicine, reinforcing Qi and promoting blood circulation to remove blood stasis medicine and Vitamin E can raise old and feeble model little mouse serum hit AchE activity effectively, and have the best result with the angry blood circulation promoting silt group of benefit. 3.Vitamin E group, reinforcing Qi group, romoting blood circulation to remove blood stasis group and reinforcing Qi and promoting blood circulation to remove blood stasis group change, improve to little mouse myocardium cell pathology of structure old and feeble to some extent, but the angry blood circulation promoting silt group of benefit is obviously superior to every other group.Conclusions Model group little mouse serum hit AchE content reduce obviously than normal group, prove, succeed in, duplicate old and feeble animal model already. The treatment of reinforcing Qi and promoting blood circulation to remove blood stasis can improve little circulation effectively, improve AchE activity in the blood, change obvious improvement function to the pathology of the myocardium cell structure, thus get the result of slowing aging. |
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