Containing Serum Of Qidantongmai Tablet To Promote Angiogenesis Research

Ischemic heart disease: due to imbalance between in coronary blood flowand myocardial demand lead to the Myocardial damage, and its increasedmorbidity and mortality rates,it become a serious threat to human health anddisease. PTCA (percutaneous coronary into the plasty), CABG (coronary arterybypass surgery) and other treatments to achieve satisfactory results, but In part of thepatients with diffuse coronary artery is not suitable for CABG or PTCA and there is highcost, complexity and restenosis after surgery and other issues. Hockel equal to 1993,presented the concept of therapeutic angiogenesis It refers to the gene by drugsor other means to promote angiogenesis in ischemic tissue, the originalmicrovessel based on new growth of capillaries, and the integration of theexisting vascular network convergence,To improve the blood supplyorganization, the compensatory ability to improve a method of myocardial.Herb play a important role of angiogenesis.TCM theory and theory-basedblood-related, combined with years of clinical practice established "Qidantongmai tablet " is an effective treatment of ischemic heart diseaseprescription, both Study found the drug to have myocardial ischemia in coronaryheart disease prevention, preventive give Qidantongmai tablet can improvemyocardial ischemia ventricular vascular endothelial growth Basic fibroblastgrowth factor (bFGF) expression [1-2] In order to clear the drug in promoting angiogenesisand improve heart function in the role, this study Qidantongmai tablets containing serumeffect on the CAM. count of blood vessels under the anatomy microscope. Analyzed byflow cytometry cell cycle umbilical vein endothelial cells. Immunocytochemicalanalysis of small cells in human umbilical vein endothelial expression of VEGFprotein to the level of tissues and cells from Yiqihuoxue medicine ofangiogenesis mechanism.ExPerimentExPerimentsⅠcontaining serum of Qidantongmai tablet on chick embryochorioallantoic membrane angiogenesisPurpose: Take containing serum of Qidantongmai tablet on the chickenchorioallantoic membrane (CAM), observed the effects on angiogenesis Method:8 SD rats were randomly divided into 2 groups, Each drug group, rats wereadministered daily for Qidantongmai tablets high dose extract powdersuspension, 3.24g / (kg ? d), 2 times a day, each 2 ml, normal serum of rats Inaccordance with normal food, water feeding, 7d after take the abdominal aortablood, centrifugedand then take the serum into -20℃refrigerator frozen Take 7days old chicken embryos, randomized, each 10, with a scalpel open the windowfor about 1 * 1cm, exposure to CAM, sterile sealing tape to hatch. The next day,take qualitative filter paper as a carrier placed on the CAM were dropping 40uLserum with normal serum in the vector control group, dropping 40uL normalsaline (NS) and bFGF, sealed incubated for 72 h, methanol and acetone mixture (1:1) at room temperature, fixed to the center carrier cut CAM, developmentvector in the filter paper, [3-5] under a dissecting microscope to count the vessel,to the edge of experiments within the range of lmm a blood vessel to the edge ofexperiments 5mm Department for two vessels were observed and analyzed to compare thenumber of angiogenesis in each group, All data use the SPSS statistical software carryon the single factor variance analysis by the even±standard deviation to compareeach group of differences ResultResult: Counted under a dissecting microscope thenumber of new vessels. Qidantongmai tablet group play a significant role inpromoting CAM angiogenesis, the number vascular of medicine group is29.17±2.99, two vessels the number of vascular is 37.83±2.71 whichcompared with NS group was statistically significant (P <0.05), but comparedwith the bFGF and promoting the role of CAM angiogenesis is relatively weak(P <0.05). Conclusion :Qidantongmai tablet play a significant role in promotingangiogenesis in CAM, which effectively improve the blood supply of ischemicmyocardium and treatment of ischemic disease may be related to the promotionof angiogenesis.ExPerimentsⅡcontaining serum of Qidantongmai tablet on humanumbilical vein endothelial cells expression of VEGFPurpose:In DMEM medium supplemented with containing serum ofQidantongmai tablets as culture medium, cultured human umbilical veinendothelial cells by immunohistochemical analysis of VEGF expressionMethod:Preparation of drug serum ,10 SD rats were randomly divided into 2groups randomly divided into drug group and physiological saline group,HUVECwill be randomly divided into 3 groups: containing serum group, normal serumgroup, negative control group, all of them were added to DMEM culture medium, containing serum, normal serum, negative control group, adding the mediumwith equal amount of PBS as control the endothelial cells were seeded in 24-wellplate in each of 4 empty join 0.82cm coverslip. Fused cells to different groupsaccording to dose, and then remove the cover slip after 24h incubation kit byimmunohistochemistry,the expression of VEGF was detected by SABCResultResult:through Microscope, a cell nucleus and cytoplasm with brown yellowgranules were positive cells, compared to normal serum containing serum groupcell borders and nuclear structure, nuclear color clearly stained, containingserum + blocker group and the VEGF negative group only weak expression.Conclusion:Qidantongmai tablet can significantly promote the expression ofVEGF, suggesting the drug may play through the promotion of VEGF expressionin ischemic angiogenesis.ExPerimentsⅢcontaining serum of Qidantongmai tablet on humanumbilical vein endothelial cell proliferationObjective: DMEM medium supplemented with Qidantongmai tablets containingserum as culture medium, cultured human umbilical vein endothelial cells andobserved the cell proliferation. Methods: 10 SD rats were randomly divided into2 groups:drug group and NS group HUVEC will be divided into 3 groups:containing serum group, normal serum group, and containing serum + blockergroup, each group were added to the culture medium, final concentration of150ML / L and were detected by flow cytometry, cell cycle, All data wereexpressed as mean±standard deviation use SPSS statistical software applicationthat single factor analysis of variance compared differences between groupsResults: Serum cultured HUVEC cycle G0/G1, phase, S phase, G2-M percentageof cells were normal. Containing serum group G0/G1 phase cells was significantly reduced (P <0.05), S phase cells was significantly increased (P<0.05) containing serum + inhibitor group than in serum compared with G0/G1phase cells increased (P <0.05) , S phase cells decreased, (P <0.05) ConclusiConclusion:Qidantongmai tablets containing drugs acting on the endothelial cells of bloodserum of each phase of the cell cycle, the number of percentage of total cellscompared with the control group were significantly different. S phase cellsincreased significantly, while the G0/G1 phase were decreased, while add Vandetanib have it analyzed by flow cytometry after cycle then found containing serumgroup compared with the phase cells G0/G1increased, S phase cells thancontaining serum was significantly decreased, (P <0.05) Description QTPMT isto promote the expression of VEGF to promote cell proliferation.cells VEGF...