Expression Of Estrogen Receptor α And β In Hepatocellular Carcinoma

Aims: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in China. The nosogenesis of HCC is related to several factors including hormone. Estrogen is metabolized and inactivated in liver, and act with each other. Cells response to hormone by receptors and receptors help to cause liver tumor and therapy with anti-hormone drugs. The study of Expression of estrogen receptor a and β in hepatocellular carcinoma will help to understand the nosogenesis of HCC. It will also help to guide the clinical therapies and judge the prognosis. Methods: 1. The examination of expression of ERa and ER β in HCCWe used immunohistochemical methods. Positive staining located mainly in the plasma and(or) nucleus. 400 cells were counted in the eyeshot of high multiply, and if the number ofpositive cells outrun 20% , we considered it positive.2. The examination of expression of ERa and ERβ in non-HBV-related and HBV-related HCC.The slides were fixed in acetone for 10 minutes in 4°C after cells culture. Then ERa and ERp were examined by immunohisto-chemical methods. In the end HPIAS—1000 system were used to analyze the results. Results:1. The expression of ERa was no significant difference between HCC tissues group and non-HCC tissues group(P>0.05), but ERβ expression in non-HCC tissues was higher than that in HCC tissues(P<0.05). ERa expression in HCC was higher than ERp expression in HCC (P<0.05) .2. The expression of ERa was no significant difference between Edmondson I and Edmondson Ⅱ,Ⅲ (P>0.05) , but ERβ expression in HCC of Edmondson I was higher than that in Edmondson Ⅱ ,Ⅲ (P<0.05). The expression of ERa and ERp had no relationship with age and gender.3. ERa and ERp expression in non-HBV-related HCC cell line was higher than that in HBV-related HCC cell line (P<0.05). Conclusion:1 . ERa and ERp exist in HCC tissues, non-HCC tissues (hepatocirrhosis, cavernous hemangioma of the liver) and normal...