Association Between Genetic Polymorphism Of Estrogen Receptor And Susceptibility To Type B Hepatitis、Cirrhosis、Hepatocellular Carcinoma

Objectives Through the investigation of the estrogen receptor gene at the polymorphism position, explore the genetic susceptibility of the ER gene and hepatitis B、cirrhosis and liver cancer.Methods High incidence area of Guangxi,107cases of healthy persons (normal group),112cases of hepatitis (hepatitis group),65patients with liver cirrhosis (cirrhosis group) and107cases of patients with hepatocellular carcinoma (liver cancer),using aggregatepolymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method and DNA sequencing were used to detect ER gene Xba Ⅰ、Pvu Ⅱ locus polymorphism,with the χ2test were normal group and hepatitis, liversclerosis group,genotype and allele frequencies of HCC difference, logstic,regression analysis,calculate odds ratios (Oddsratios, OR) and95%confidence interval (the Confidence for Intervals, CI),by sex and age correction of ERgene polymorphisms with hepatitis,cirrhosis,liver cancer, genetic predisposition for association analysis using PLINK software to build haplotype and analyzed.The above statistics using SPSS13.0software.Results1. A normal group and the hepatitis group and the cirrhosis group,the liver cancer group differences in gender and age composition,no significant (P>0.05)were comparable.Normal group,group of hepatitis,liver cirrhosis group,liver cancer Xba Ⅰ、Pvu Ⅱ of genotype are in line with the Hardy-weinberg balance detected in the sample is representative and comparable.2.The Xba Ⅰ sites of AA,AG and the GG genotypes,compared with the normal group,AG genotype in the hepatitis group of cirrhotic patients,liver cancer,the frequency distribution of the difference was not statistically significant(P=0.785,P=0.884,P=0.722),the GG genotype in the hepatitis group, cirrhotic patients,liver cancer,the frequency distribution of the difference was statistically significant(P=0.566,P=0.301,P=0.331),the G allelehepatitis group, the cirrhotic patients,liver cancer group,the frequency distribution of the difference was not statistically significant(P=0.977,P=0.227,P=0.499).AG genotype compared with the AA genotype,and hepatitis, cirrhosis,liver cancer risk of no correlation(P=0.785,P=0.884,P=0.722),the GG genotype compared with AA genotype,and hepatitis,cirrhosis,liver cancer risk (P=0.566,P=0.301, P=0.331),indicating the genetic susceptibility of the Xba I sites of gene polymorphisms with hepatitis,cirrhosis,liver cancer nothing to do.3.Pvu Ⅱ sites of the total of the TT,TC and CC genotypes,and the normal group compared with the TC genotype frequency distribution in the hepatitis group,the cirrhotic patients,liver cancer group difference was statistically significant(P=0.046,P=0.028,P=0.032),the CC genotype in the hepatitis group, the cirrhosis group,liver group,the frequency distribution of the difference was statistically significant(P=0.044,P=0.043,P=0.010), C allelehepatitis group,the cirrhotic patients,liver cancer group, the frequency distribution of the difference was statistically significant (P=0.010,P=0.005,P=0.001).TC genotype compared with the TT genotype and the risk of hepatitis,cirrhosis,liver cancer correlation(P=0.046,P=0.028,P=0.032),compared with the TT genotype,TC genotype suffering significantly increased to1.41times the risk of hepatitis,the risk of suffering from cirrhosis of the liver was significantly increased to1.35times,the risk of liver cancer was significantly increased to1.31times,the TC genotype may be a genetic susceptibility gene of hepatitis,cirrhosis,liver cancer,.CC genotype and hepatitis,cirrhosis,liver cancer risk was correlated (P=0.044,P=0.043,P=0.010), compared with the TT genotype, the CC genotype was suffering from hepatitis risk was significantly increased to1.76times,the risk of suffering from cirrhosis of the liver was significantly increased to1.92times,the risk of liver cancer was significantly increased to1.75times,indicating that the CC genotype may be a genetic susceptibility gene of hepatitis, cirrhosis, liver cancer.4.Compared with the normal group,Beijing,the Japanese,Utah residents and Nigerians of Xba I sites of genotype and allele frequency distribution have the similar results,at Pvu Ⅱ of sites of genotypes and allele frequency distribution findingsquite different.5.AC haplotype in the cirrhosis group and the frequency of liver cancer was significantly higher than the normal group, the AC haplotype risk of suffering from liver cirrhosis increased to1.22(P=0.006,OR=1.216), the risk of liver cancer increased to1.46(P=0.003,OR=1.456), suggesting that the AC haplotype may be a predisposing factor for cirrhosis, liver cancer. Haplotype and disease risk (P>0.05). Conclution1.ER gene at Pvu Ⅱ of sites the TC and CC genotypes have the genetic susceptibility with hepatitis, cirrhosis,liver cancer, the TC genotype suffers from hepatitis risk was significantly increased to1.41times the risk of suffering from cirrhosis of the liver was significantly increased to1.35times, suffering fromsignificantly increased to1.31times the risk of liver cancer; CC genotype was suffering from hepatitis risk was significantly increased to1.76times the risk of suffering from cirrhosis of the liver was significantly increased to1.92times the risk of liver cancer was significantly increased to1.75times, indicating that the TC, CC genetype may be a genetic risk factor for hepatitis, cirrhosis, and liver cancer.2.Xba I genotype and allele frequencies in Beijing, Japan, Utah, USA and Nigeria, the results were similar at Pvu II of genotype and allele frequencies and Beijing, Japan, Utah, USA and Nigeriato the findings of differences.3.AC Single-fold risk of suffering from liver cirrhosis increased to1.22, the risk of liver cancer increased to1.46, suggesting that the AC haplotype may be a predisposing factor of liver cirrhosis, liver cancer.