The Expression Of GPR30,ERα,ERβ,PR, VEGF In Endometrial Carcinoma And Its Significance In Endometrial Carcinoma

Objective To investigate the expression of G protein-coupled estrogen receptor、vascular endothelial growth factor^ estrogen receptor alpha、estrogen receptor beta and progestrone receptor in endometrial carcinoma、atypical hyperplasia endometrium and normal endometrium, and to explore their correlation in endometrial adenocarcinoma and the relationship with the clinical-pathological features, to analysis the role in the pathogenesis and development, we expected that the study will help us to Help individual comprehensive therapy of tumor.Methods The expression of GPR30、ERα、ERβ、PR and VEGF in 31 cases of normal endometrium,37 cases of endometrial atypical hyperplasia and 93 cases of endometrial adenocarcinoma tissues were detected by immunohistochemical method. The statistical analysis methods of data:the count materials were evaluated with χ2 test or Fisher probabilities in 2x2 table; the correlation using Spearman rank correlation analysis;Results The GPR30 and VEGF expression showed an ascending tendency, with significant differences among normal endometrium, endometrial hyperplasia and endometrial carcinoma groups (P<0.05). The differences of the positive expression of PR and ERβ between endometrial atypical hyperplasia and endometrial adenocarcinoma, normal endometrium were not statistically significant (P> 0.05). The high GPR30 expression was significant correlation with the poor differentiation, high TNM staging, lymphatic metastasis (P<0.05). The expression of PR in endometrial adenocarcinoma is related to their histological differentiation and clinical stage. The expression of VEGF in endometrial adenocarcinoma was related to clinical stages (P<0.05)and lymph node metastasis (P< 0.05), but not related to cancer differentiation (P> 0.05). The expression level of GPR30 was negatively correlated with VEGF (P<0.05), And was no relationship with ERβ and PR (P> 0.05). VEGF expression was significantly correlated with ERain the endometrial cancer tissue with expressing GPR30 (P<0.05). the expression of ERα、ERβ、and VEGF and PR had positive correlation. the expression of GPR30 and ERahad positive correlation and no relationship with ERβ.Conclusion 1. ER alpha and GPR30 play a key role in development and incidence of estrogen dependent endometrial carcinoma, PR and VEGF play a key role during the progress of tumor; 2. In the process of endometrial cancer cell differentiation, ER alpha and PR may play a role, while GPR30 may inhibit the differentiation of endometrial cancer cells; 3. GPR30 and VEGF in endometrial cancer associated with infiltrating invasion and lymph node metastasis, and the expression of VEGF protein and GPR30 expression were positively correlated in endometrial carcinoma.We concluded that estrogen may regulat the expression of VEGF which promoted the new tumor angiogenesis through the GPR30 non genomic effects, then promotes the development of tumor, invasion and metastasis in endometrial;4. VEGF expression was significantly correlated with ERαin the endometrial cancer tissue with expressing GPR30.We concluded ER alpha may compete with GPR30 through the combination of estrogen, then decreased estrogen effect of genome pathway, at the same time reduce the production of VEGF, inhibit tumor growth, invasion and metastasis;5. The expression of ER alpha in endometrial carcinoma and PR expression may influence each other, at the same time ER alpha、GPR30 expression and influence each other.But the specific mechanism needs to be studied further.