| Objective: In order to probe the essence of blood stasis condition of chronic bronchitis with deficiency of lung-energy from view of microcosmic, then further to find the functional mechanism of strengthening QI to remove blood stasis Chinese medicinal herbs (comparing with traditional replenishing and restoring lung-energy herbs), We detected contents of endotheins (ET-1) , nitrogen monoxide (NO) , hromboxane (TXB2), and 6-keton-prostaglandin dinoprostone (6-Keto-PGF1α) in blood and lung tissue,and observed the expression of vascular endothelial growth factor (VEGF) in bronchus and lung tissue of chronic bronchitis rats with deficiency of lung-energy.Methods: 50 SD rats were fed in experimental environ- ment one week before experiment, room temperature was 20℃~25℃. SD rats were randomly divided into two groups, 10 rats were in normal control group (which was abbreviated to "normal group" in the following) and 40 rats in patternmaking group, male and female was half respectively. Patternmaking group was duplicated for the animal model of deficiency of lung-energy of chronic bronchitis. The rats in patternmaking group were placed in a 1m×1m×1m smoking-fumigated room, smoked with wood shaings, sawdust and tobacco leaf, 50g respectively, twice a day, 30 min once, lasting for 30 days. The rats in normal group were fed in a room without smoke. The weight of rats was weighted at 1, 8, 15, 22 and 30 days, and general condition of rats was observed. After model were produced successfully, patternmaking group rats were randomly divided into four groups: model control group (which was abbreviated to "model group" in the following) , high-dose strengthening QI to remove blood stasis group (which was abbreviated to "high-dose group" in the following) , low-dose strengthening QI to remove blood stasis group (which was abbreviated to "low-dose group" in the following) , buqi control group (which was abbreviated to "buqi group" in the following, tradition herb of replenishing and restoring lung-energy) . There were 10 rats in each group, and male and female was half respectively.From the second day after model were successfully produced , 5 groups were continuously poured medicine or saline into stomach for 21 days. Normal groups were poured saline 10ml/kg, 1 time a day; model group were poured saline 10ml/kg, 1 time a day; high-dose group were given medicine 15.6g/kg (14 times as much as clinical adult dosage) and poured 1 time a day; low-dose group were given medicine 7.8g/kg (7 times as much as clinical adult dosage) and poured 1 time a day; buqi group were given medicine 7g/kg (7 times as much as clinical adult dosage) and poured 1 time a day.The second day after treatment all rats were cut head for obtaining blood, which were centrifugated for 5 minutes (3500r/min) at 4℃ immediately, parted blood plasma and taked blood serum. We saved blood plasma and blood serum to freeze at -20℃ for check up. We weigh out a piece of 0.3g wet tissue of left lung and flushed it by freezed saline and sucked water on it by filter paper. Then put it in prepared cold saline. We used the electric homogenizer to dispense the wet tissue into 10% lung tissue homogenate, centrifugated for 5 minutes (6000r/min)at 4℃, and extracted the above fluid to save freezed at -20℃ for check up. We took an area of 0.5cm×0.5cm from right lung tissue, which were fixed by 10% formaldehyde solution. The tissue section was stained by immunohistoche- mistry. We used radioimmunoassay to detect the content changes of ET-1TXB2,6-Keto-PGF1αin blood plasma and lung tissue. Used nitrate reductase method to detect the content changes of NO in blood serum and lung tissue. Used immunohistochemis- try dyeing method to observe the expression of VEGF in bronchus and lung tissue.Results 1 general condition: 12 days after smoking-fumigated the rats in patternmaking group showed the symptoms, such as respiratory difficilty, cough, lassitude, losing brightness of hair and so on; while the rats in normal group we...
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