Expression Of Midkine In Gallbladder Carcinoma

BackgroundThe procedure of gallbladder carcinoma's episode is secretiveness. Gallbladder carcinoma's invasiveness is powerful, and its prognosis is poor. Because of the deficiency of early period diagnosis method and the powful of invasiveness, gallbladder carcinoma therapeutic efficacy is very poor. In recent years, great progress has received about gene research. Many correlation genes of gallbaldder carcinoma had been found. With the progression of molecular biology, it is possible to improve gallbadder carcinoma curative effect.Midkine is a new heparin-bingding growth/differentiation agent.lt was first distinguished as a product of gene induced by retinoic acid. Four biologic functions were confirmed at present. It promotes embryo cranial neiraxon outgrowth. It increases plasm zymogen activity of aorta endothelial cell. In nude mouse ,it makes NIH3T3 cell carcinomatous change. It promotes mitosis and angiogenesis. Oversea research also indicated that Mikdine had a role of anti-apoptosis in Wilm's tumor and embryo nerves. Midkine also facilitates transfer of embryo nerves cell, neutrophil and macrophage. These two function may help to tumor cell soakage and survive. High expression of Midkine has related to clinical prognosis in neuroblastoma, gliocytoma and bladder carcinoma. The expression of high differentiation gastric carcinoma cell is lower than low differentiation one and signet-ring cell carcinoma. In adult tissue,low expression of midkine was detected in kidney,lung,stomach,colon,ovary and thyroid gland. But hig expression of Midkine was detected in human malignancy(breast cancer,lung cancer,esophageal carcinoma, gastric carcinoma, ovary carcinoma,bladder carcinoma,gliocytoma,neuroblastoma and Wilm's tumor.At present,there is know few about Midkine expression in the carcinoma of gallbladder. We still don't know what is role of Midkine in the gallbladdercarcinomatous change and development. In this experiment, we will comprehend and research clinical significance of Midkine by detecting the expression of Midkine in the gallbladder carcinoma . In order to comprehend much more , we will compare the expression of midkine to nm23,Ki67and CD34 in gallbladder carcinoma.Objective1. To investigate the expression of Midkine in human gallbladder carcinoma and its significance in cancer biological feature.2. To explore the relation expression of Midkine to gallbladder carcinoma's angiogenesis, cellproliferation and prognosis.Methods1. 53 cases of gallbladder carcinma,12 cases of metastasis lymph node, 12 cases of abdominal metastasis point ,14 cases of adenoma,20 cases of inflammatory polyp,20 cases of cholecystitis constitution and 18 cases of normal gallbladder were reviewed in this study. Expression of Midkine was evaluated by immunostaining with Midkine polyclonal antibody.2. Various kinds of gallbladder lesion expression of Midkine was evaluated by immunostaining with Midkine polyclonal antibody.3. Expression of Ki-67 ,nm23 were evaluated immunohistochemically with Ki-67and nm23 monoclonal antibody in gallbladder carcinma metastasis lymph node abdominal metastasis point adenoma cholecystitis constitution and normal gallbladder. Microvessel density (MVD) was examined by immunostaining with CD34 monoclonal antibody. They were compared to the expression of Midkine respectively.4. Labelling index(LI); Microvessel density .5. Statistical analysis methods: Wilcoxon rank sum test and t check. All of the data was analysed by SPSS9.0 software.Results1. Midkine is expressed in most cells of gallbladder carcinoma gallbladder carcinoma. LI of the expression of Midkine is 72 (41-98) .2. LI of the expression of Midkine of normal gallbladder, inflammatory polyp, cholecystitis constitution, adenoma and gallbladder carcinoma is 0,5(0-12), 7(0-16) , 17(5-29) and 72(41-98). There is significant differential expression between the gallbladder carcinoma and other gallbladder constitution(P<0.01 ) .3. There were relations between the ex...