| Focal segmental glormerulosclerosis (FSGS) has a morbility of approximately 10%~20% in childhood original nephrotic syndrome (NS)and is common in adult and childhood end stage renal failure. Though FSGS has been described by Fahr in 1925 FSGS progression mechanism is still mistiness. Because of long clinical course and dangerous prognosis FSGS is still a difficult problem in nephrosis field. To improve curative effect, we carried out the study.Objective: To establishes focal segmental glormerulosclerosis (FSGS) the model, discusses FSGS the treatment.Methods: thirtyfive male Wistar rats were divided into seven groups: normal control group(A,n=5),shenmai-treated group(B,n=6),heparin-treated group(C, n=6) , benazepril-treated group(D,n=6),integrated treatment group(D,n=6), and model group(F, n= 6).Rat model of FSGS was constructed with low-dose adriamycin, metaraminol and high lipid feed method. All treatment group were treated at 29th day . 24-hour urinary protein was measured at the 0, 2nd, 4th, 6th and 12th week. Serum total protein, album in, cholesterol, blood urea nitrogen(BUN)and serum creatinine (SCr) were determined at 0, 2nd, 6th and 12th week. Renal cortex blood flow was performed at the 12th week .Renal pathology changes were evaluated at the 12th week as well.Results Model group:24-hour urinary protein was increased at 12th week. The serum cholesterol leve was maximum at 2th week.2.Treatment group: 24-hour urinary protein were decreased in treatment group at 2th week and were significantly lower than that in the model group at 12th week. The serum cholesterol level in treatment group was lower than that in model group at 12th week. 3.Renal cortex blood flow was increased significantly integrated treatment group. Glomerular sclerotic index(SI)and glomerular extracellular matrix(ECM )/glomerular area(GA) weredecreased significantly in integrated treatment group.Electron microscopy revealed that there was no glomerular capillary cavities obstruction in integrated treatment group, basement membrane change and foot precesses fusion were markedly alleviated in comparison with model group.Conclusion 1. Rat model of was constructed with low-dose adriamycin, metaraminol and high lipid feed method and that the FSGS model is a new model for renal disease. It is first time to confirm shenmai injection effect on FSGS and shenmai, heparin and bennazepril can be used as basic drugs.Itegrated shenmai, heparin and benazepril may be play a valuable role in the prevention and treatment of FSGS from more targets. |
