| Objective:(1)To investigate the expression of TGF-β1 and VEGF in kidney tissue of FSGS rats and the role of two factors in the development of FSGS.(2)To observe the effects of Rosiglitazone of FSGS rats and the possible role of the mechanism.Methods:22 male Wistar rats were randomized to three groups:normal control group(A,n=6), model group(B,n=8)and Rosiglitazone-treated group(C,n=8).Rats were left-nephrectomized,then injected 1%Adriamycin caudal-venously by 5mg/kg and 3mg/kg respectively.The rats in normal control group were sham operated,and were injected normal saline with the equal volume at the same time.Rosiglitazone-treated group was treated at 29th day of the operation for 4 weeks.24-hours' urinary protein and blood biochemical indicators were measured at the 0,4th,6th and 8th week.At 8th week all rats were killed,the left kidney was obtained for pathology observing.Ccr,SI and ECM/GA were calculated.Renal histopathological lesions were evaluated by the counting score method.Expressions of VEGF and TGF-β1 were detected by immunohistochemical method.Results:(1)In the 4,6,8-week,compared with the normal group,model and rosiglitazone treatment of rats 24 hUP,Cho,BUN have increased significantly,there were significant lower in serum TP,Alb and Ccr.(2)In six weeks,compared with the model group,there were significant lower in serum Cho and serum TP were significant elevated,differences were statistically significant(all P＜0.05);In eight weeks,compared with the model group,Romania Comparison of rats in the treatment of 24 hUP and Cho are lower,there were significant differences(P＜0.01,P＜0.001)serum TP,Alb and Ccr have increased,had a significant difference(P＜0.01,P＜0.01,P＜0.001).2.Light microscopic observation result showed that:in model group,part of glomerular hypertrophy and hyperplasia of mesangial matrix,balloon adhesion,a hardening of the capillary loop change,we can see that tubular swelling,and protein denaturation of the type stove with infiltration of inflammatory cells and interstitial fibrosis.Glomerular sclerotic index(SI)and glomerular extracellular matrix(ECM)/glomerular area(GA)of Rosiglitazone-treated group was lower than model group.Renal histopathological lesions of Rosiglitazone-treated group was less than model group.3.(1)The expression of TGF-β1 was the highest in model group and the lowest in control group in glomerular area.The expressions of Rosiglitazone-treated group was decreased than that of model group.Tubulointerstitial area expression of TGF-β1 was weakly positive,the group with no significant difference.(2)Glomeruli of rats in each group only trace expression,the difference was not statistically.VEGF expression region mainly concentrated in the tubulointerstitial area.Compared with model group,rosiglitazone treatment group decreased.4.TGF-β1 and glomerulosclerosis index(SI),matrix area ratio(ECM/GA)and glomerular lesions score were positively correlated(r=0.869,p=0.025,r=0.828,p =0.042,r=0.975,p=0.001);VEGF and renal interstitial lesion score positively correlated(r=0.824,p=0.044).Conclusion:1.Unilateral nephrectomy plus points of the tail vein injection of doxorubicin for us to study how to prevent or delay the progress of FSGS provides a small difference between the groups,stability and repeatability good experimental animal models.2.Expression of TGF-β1 in glomerular excessively participate the promotion of focal segmental glomerulosclerosis and the pathological changes.The kidney TGF-β1 level may be used as a sensitive indicator of FSGS.3.Vascular lesions is one of the important factors for FSGS progrece.4.Rosiglitazone has a protective effect.It can reduce proteinuria in rats,reducing infiltration of inflammatory cells,reduce TGF-β1 and VEGF expression,reduce glomerulosclerosis,renal fibrosis of the kidney in FSGS rat. |
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