Protective Effect And Mechanism Of Yishen Huoxue Recipe On Podocyte Injury In Rat Model Of FSGS

Objective:Through the experiment,we observed the protection of Yishenhuoxuefang on adriamycin nephrosis(ADN)model of podocyte,and explore the pathogenesis of FSGS.Methods:Using a randomized control method,the animal models were prepared by using the method of tail intravenous injection of adriamycin(4mg/kg/once)twice.The second tail intravenous interval two weeks,and the 24h U-Pr was measured on the 7 and 14 day after the second tail intravenous.If the 24h U-Pr>100mg/24h,means the success of making model,raising them for 8 weeks and measured the 24h U-Pr.Eliminated the failure of the model,the tail fester,or the death rat,the remaining 34 model rats were included in the experiment.The rats were randomly grouped into model group?captopril group?low dose group of Yishenhuoxuefang and high dose group of Yishenhuoxuefang based on the final 24h U-Pr and weight.With the another blank group,we had five groups.Then gave them eight weeks treatment respectively.In the process of experiment,on the one hand,we observed the rats between each detection rat body weight,24h U-Pr,ALB,Scr,BUN.On the other hand,gave the kidney histologic examination of HE and PAS staining;What is more,using Western blotting method for detection the expression level of WT1 and using RT-PCR for detecting podocin and nephrin mRNA expression of the rat kidney;All the data analysis processing via the software of SPSS 20.0.Results:?The physical status and symptom of the glomerular sclerosis rats were improved obviously after treating by Yishenhuoxuefang.?On the weight,8 weeks after module building,there was a statistically significant(P<0.01),compared with the blank group;After drug intervention,high dose group and the captopril group still had weight gain and there was a downturn in the model group since the 12 weeks.At the end,the high dose group and captopril group expressed statistically difference between the model group(P<0.05).?In view of the Urinary protein,there was a significant statistically difference between the low dose group of Yishenhuoxuefang and model group(P<0.01),and there was a statistically difference between the captopril group?Yishenhuoxuefang group and model group(P<0.05).?On the ALB,high dose group and captopril group had improved,and no statistically difference compared with the blank group(P>0.05)after treatment.?From the renal function of Scr,high dose group improved the most obvious,with a significantly statistical difference of model group(P<0.01),and a statistical difference of low dose groups(P<0.05);when it comes to BUN,western medicine group improved the most obvious;the high dose group and captopril group expressed a statistically difference between the model group(P<0.05.?On the histopathology,glomerular sclerosis index,ratio of matrix was improved after treatment,although statistically significant compared with the blank group(P<0.01),but statistically improved with the model group(P<0.05).?When it comes to WT1,the model group had a obvious drop.There was a statistically difference between the captopril group?Yishenhuoxuefang group and model group(P<0.05).From the nephrin and podocin mRNA,there was a significant statistically difference between the captopril group?Yishenhuoxuefang group and model group(P<0.01),and there was no difference between groups respective.Conclusions:Yishenhuoxuefang can improve the hypoalbuminemia,proteinuria and renal function of FSGS in rats,and reduce the degree of glomerular sclerosis;also its mechanism may be related with the increased expression of WT1 protein and nephrin,podocin mRNA.