The Correlation Between Platelet-derived Microparticle And Chronic Renal Failure Of Type 2 Diabetes

Objectives The study gave the research to the correlation between platelet-derived microparticle(PDMP) and chronic renal failure of Type 2 Diabetes. Methods The study collected altogether 93 objects. The objects were divided into there groups according to the patients with diabetes and without diabetes and their creatinine. Group 1:Type 2 diabetes with abnormal creatinine. Group 2: Type 2 diabetes with normal creatinine. Group 3: Normal glucose tolerance and without abnormal creatinine. It collected general clinical data, checked blood routine and item of biochemistry, prepared the platelet-rich plasma,and then check the CD62p and CD42a by flow cytometry. CD62P-FITC monoclonal antibody was used to label activated platelet and CD42a-PE monoclonal antibody was used to label PDMP. All parameters were analyzed by SPSS 11.0 software. Results (1)Analysing CD62P with one way ANOVA, we could come to the results: at least two groups of the three were different significantly. Then did q test: group 1 vs group 2, p<0.05, differently; group 2 vs group 3,p<0.05, differently; group 1 vs group 3,p<0.05,differently.(2)Analysing CD42a with one way ANOVA, we could come to the results: at least two groups of the three were different significantly. Then did q test: group 1 vs group 2, p<0.05, differently; group 2 vs group 3, p<0.05, differently; group 1 vs group 3, p<0.05,differently.(3) Simple correlation showed CD62P correlated with CD42a(P<0.01), urea nitrogen(P<0.01), creatinine(P<0.01), systolic pressure(P<0.01) and hemoglobin(negativecorrelation,P<0.01).CD42a correlated with CD62P(P<0.01),urea nitrogen(P<0.01), creatinine(P<0.01) and systolic pressure(P<0.01). Stepwise multiple linear regression analysis suggested the independent risk predictor for CD62P were CD42a and hemoglobin, and the independent risk predictor for CD42a were CD62P and creatinine.Conclusions (1)Activated platelet correlated with PDMP. (2)CD62P and CD42a of patients with diabetes were higher than that of patients without diabetes. CD62P and CD42a of patients with Type 2 diabetes and abnormal creatinine are higher than that of patients with Type 2 diabetes and without abnormal creatinine. It illustrates actived plateles and PDMPs of the patients with Type 2 diabetes were significant higher than patients without diabetes. The case was Type 2 diabetes with abnormal creatinine and Type 2 diabetes with normal creatinine.(3)PDMPs correlated with creatinine of the patients with Type 2 diabetes. Urea nitrogen, creatinine and systolic pressure were the predictors for worsening PDMPs. The results suggest that PDMPs may participate in the development of chronic renal failure in patients with diabetes.