Experimental Study On Anti-asthmatic Effect Of Modified Zhi Sou San And Its Mechanism

Objective: To offer the gist of controlling Modified Zhi Sou San's (MZ's ) quality, and to study the anti-asthma anti-tussive expectorant anti-inflammatory effects and its mechanism.Methods : The active parts having anti-asthma anti-tussive expectorant anti-inflammatory effects were selected by the activity extract method with phytochemistry and pharmacology. Models of anti-tussive experiments in mice with cough were induced by ammonia water and expectorant experiments were used the mice with phenol red excretion method . The inflammatory models were caused by dimethylbenzene. Bronchial spastic asthma was induced by histamine and acetylcholine in guinea pigs and the model of sensitized guinea pigs were caused by egg albumin. The anti-asthma mechanism was observed by isolated tracheas, lung tissue and other cytokine. Results: Modified Zhi Sou San was divided two parts. The low fat-soluble part was called MZS and the high fat-soluble part was called MZC. MZC could prolong their cough latent period and decrease their cough frequency, could increase secretion of phenyl red in the airway in mice, could prolong latent period of bronchial asthma in guinea pigs and relieve the mouse ear swelling remarkably. Under the light microscopy pathological changes in the lung tissue were obviously lighten. The content of MDA was reduced remarkably in serum and lung tissue, while the activity of SOD was elevated significantly. The number of eosinophilias and the contents of ET-1 and NO in the BALF and blood were descended remarkably. MZC could increase weights of thymus and spleen of mouse to certain degree. It could adjust the level of IL-4 and IFN- γ in supernatant of mouse spleen cells. It could reduce isolated tracheas contraction caused by acetylcholine and histamine.Conclusion: MZ had cough relieving, expectorant, anti-inflammatory and anti-asthma effects. MZC was MZ's effective part. The mechanism by which MZC anti-asthma may be related to reducing the bronchial contraction and descending the number of EOS and adjusting the ratio of Th1/Th2 and ET-l/NO and increase the activity of SOD . MZC could enhance anti-inflammatory and antioxidant effects.