| Objective: To build the experimental animal model of Cold-stagnate and Blood - stasis - syndrome in type 2 diabetes mellitus, in order to regularize and standardize the TCM animal model of type 2 diabetes mellitus. Methods: 80 mice were randomly dividedinto four groups: normal control group, high fat intake group, diabetes mellitus blank group and Cold - stagnate and Blood - stasis -Syndrome group (built by Traditional Chinese herb Huanglian and Shigao). Then the model group were treated by Traditional Chinese herb Xue Fu Zhu Yu Tang. Results: 1 The triglycerides, cholesterole and low density lipoprotein in model group is obviously higher than that in normal control group and high fat intake group (p < 0. 01). 2 The cortisol level in model group is obviously higher than that in normal control group (p < 0. 01). 3 FT3 in model group is obviously lower than that in normal control group, high fat intake group and diabetes mellitus blank group (p < 0. 01). 4 The insulin level in model group is obviously lower than that in normal control group, high fat intake group and diabetes mellitus blank group (p<0. 01). 5 The thyroxin B2 in model group is obviously higher than that in normal control group, high fat intake group and diabetes mellitus blank group (p<0. 01). 6 The 6-keto prostaglandinja in model group is obviously lower than that in model group, high fat intake group and diabetes mellitus blank group. 7 All the index signs were instaurated in modification group. And there was obviously differences between modification group and model group in triglycerides, cholesterole, thyroxin B2 and 6-keto prostaglandinia. Conclusion: Traditional Chinese herb Huanglian and Shigao can be used to built the animal model of Cold-stagnate and Blood - stasis - syndrome in type 2 diabetes mellitus.
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