| [Object] Observe the vary regulation of the gene of Nogo-A and BDNF, Exlore the effects of Nogo-A and BDNF-mRNA on brain after damaged . [Methed] In the present study, in situ hybrdization, tar purple stain were used in a focal cerebral ischemia- Reperfusion model with middle cerebral artery occlusion in 36 health adult Wistar rats to examine the expression profile of BDNF and Nogo-A[Result] In low magnification of tar purple stain, neuron in central area necrosis and in semi-sepheral area decreased and degeneration: cell body was triangle, sector, long-strip, with karyopyknosis, deep staining of nucleolus, and neoplasm was vacuoles. Cortex neuron was neoplasm-rarefaction, nuclea-malformation slightly and deep-staining. Degenerated neurons and normal neurons were coexist. There were lots of neurogangalions in both the cetral area and semi-sepheral area. Our results also indicated that the expression of Nogo-A-mRNA in the cerebral cortex was increased after cerebral ischemiae compared with pseudo-operation. There showed the first peak within 12 hours, decreased in 24h, and then the second peak in 48h, declined gradually, reached the level of background from 7d to 14d. while its expression in striatum increased firstly in 2h, decreased gradually to background level in 24h, and then alsothe second peak in 48h, finally reached to background level from 3d to 14d. The expression of BDNF in the cerebral cortex was increased after cerebral ischemia compared with pseudo-operation, reached to the only peak in 24h, and then decreased gradually to the background level afterwards. While its expression in striatum reached to the only peak in 2d, 3d. Then decreased gradually to the level of ischemia in initial stage. The expression of BDNF and Nogo-A in striatum was lower than in the cerebral cortex. [Conclusion] The expression of BDNF and Nogo-A all increased apparently in brain injury, they were related to the re-prosthetic of nevous tissue after damaged.
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