| Objective:By observing the expression of Nogo-A and Ng R protein in the cerebral ischemic area of rats after cerebral ischemia,we can judge the effect of acupuncture on axon regeneration after cerebral ischemia,and then explore the mechanism of axon regeneration after cerebral ischemia,so as to provide theoretical and experimental basis for the clinical application of electroacupuncture in the treatment of cerebrovascular diseases.Methods:Thirty Sprague Dawley rats,male,weighing 260 ± 40 g,were healthy adults.The rats were randomly divided into two groups: sham operation group and model replication group,with 6 rats and 24 rats in each group.In the model group,the modified longa method was used to establish the right middle cerebral artery occlusion(MCAO)model.Rats in the sham operation group were bound in the same way as those in the model group,but no model was made.The MCAO rats were randomly divided into two groups: Electroacupuncture group and ischemia group.Baihui(Baihui),Yintang(Yintang)and Zusanli(Zusanli)acupoints were selected to intervene the rats in the electroacupuncture group after ischemia-reperfusion injury.The rats were treated once a day for two weeks.The rats in the sham operation group and the ischemia group were only bound daily without electroacupuncture intervention.Two weeks later,longa model was used to evaluate the experimental rats,nerve function defects were evaluated,and the behavioral indexes were detected by vertical screen test.The expression of Nogo-A and Ng R protein was detected by Western blot and immunohistochemistry.The CO staining of Nogo-A and Ng R was observed by immunofluorescence.Result:1.Nerve function score 72 hours after cerebral ischemia-reperfusion: the nerve function score showed that the nerve function damage of the model group was significantly higher than that of the sham operation group(P < 0.01).After 14 days of electroacupuncture treatment,the nerve function score of the electroacupuncture group was lower than that of the ischemic group(P < 0.01),and there was no significant difference between the electroacupuncturegroup and the sham operation group(P > 0.05)2.Behavioral test of rats in each group after cerebral ischemia-reperfusion: compared with the sham operation group,the retention time of rats in the ischemic group was significantly reduced(P < 0.05);compared with the ischemic group,the retention time of rats in the electroacupuncture group was higher than that in the ischemic group(P < 0.05).3.Western blot showed that the expression of Nogo-A and Ng R in the ischemic area of rats in the ischemic group was higher than that in the sham operated group(P < 0.05);the expression of Nogo-A and Ng R in the ischemic area of rats in the electroacupuncture group was lower than that in the ischemic group(P < 0.05).4.Immunohistochemical results showed that the expression of Nogo-A and Ng R positive cells in the ischemic group was significantly higher than that in the sham operated group(P < 0.05);the expression of Nogo-A and Ng R positive cells in the electroacupuncture group was significantly lower than that in the ischemic group(P < 0.05).5.The results of immunofluorescence showed that the intensity of Nogo-A and Ng R in the ischemic group was significantly higher than that in the sham operated group(P < 0.05);the intensity of Nogo-A and Ng R in the electroacupuncture group was significantly lower than that in the ischemic group(P < 0.05).Conclusion:1.Electroacupuncture at Yintang,Baihui and Zusanli points can significantly improve the neurological deficit of rats with cerebral ischemia-reperfusion injury.2.Stimulation of Baihui,Yintang and Zusanli points by Electroacupuncture can improve the behavioral ability of rats.3.Electroacupuncture at Baihui,Yintang and Zusanli points can protect the nerve function and regeneration by down regulating the expression of Nogo-A and Ng R,inhibiting the apoptosis of nerve cells and avoiding the further injury of nerve. |
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