| The model of persistent infection of vascular endothelial cells with CVB3 in vitro was established. The persistently infected ECV3(M have been maintained for over 45 passages (ISOdays) and have released virus continuously without cellular destruction. Infectious center assays showed only 1-2% cells released CVB. Viral antigen could be detected. Inherent ability of WBC didn' t change. The CVB3' s genes could be detected in ECV3(M by RT-PCR. CVB3 can persistently infect ECV30< in vitro, suggesting that endothelial cells may consititute a reservoir for long-term virus production and therefore be especially relevant to the development of chronic diseases. |
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