Experimental And Clinical Studies On Erzhitiangui Decoction In Improving Quality Of Oocytes Of In Vitro Fertilization And Embryo Transfer (IVF-ET) Program

Objective: To study the effect of Erzhitiangui(EZHTG) decoction in improving quality of oocytes of IVF-ET program, and explore its acting mechanism. Methods: Experimental study : Eighty rats were randomly divided into four groups, the 1st, 2nd, 3rd and 4th group were respectively treated with EZHTG decoction combined with HMG, alone EZHTG decoction, alone HMG and 0. 9% brine. Ovulation and splitting ovums were observed to study the effect of EZHTG decoction to quality of oocytes. Clinical study: Thirty-three patients of sterility were randomly divided into the tested group(EZHTG decoction combined with western medicine) and the control group (western medicine), which were observed on improvement of symptoms of TCM before and after treatment and detected serum estradiol level on the day of injecting HCG. We compared the grades of oocyte and embryo morphology, fecuudation rate, splitting ovums rate and pregnancy rate of the two groups. Results: Experimental study indicated that the groups of EZHTG decoction combined with HMG and alone HMG have no differences in the effect of ovulation(P>0. 05), but the former was better than the later in the grades of embryo morphology, fecuudation rate, splitting ovums rate(P < 0. 05). Clinical study showed the tested group was obviously better than the control group in improving the insufficiency of kidney (P<0. 05). As compared with the control group, serum estradiol level of the tested group on the day of injecting HCG dropped appreciably, but had no differences(P > 0.05). This suggested the tested group had the tendency of decreasing theoccurrence of OHSS . As to the grades of oocyte and embryo morphology, fecuudation rate, splitting ovums rate and pregnancy rate, there were statistically significant differences between the two groups(P<0.05). Conclusion: EZHTG decoction had cooperation with western medicine on ovulation induction. This project not only assured the effect of ovulation induction but also elevated quality of oocytes, fecuudation rate, splitting ovums rate and pregnancy rate. The effective mechanism may have relation to the integral regulating function of Chinese herbal medicine which can promote the upgrowth of oocytes,adjust immune function, improve the level of reproductive endocrine, quality of oocytes and endometrial receptivity.