Morphologic Study On NPY-immunoreactive Neurons In Cardiovascular-regulation Center Of Atherosclerotic Rabbits

Objectives (1)To observe the morphologic changes of the NPY-immunoreactive neurons in cardiovascular-regulation center(CG, ARC and RVL) of atherosclerotic rabbits. (2)To observe the morphologic changes of the NPY-immunoreactive neurons in cardiovascular-regulation center of the atherosclerotic rabbits which VitE was administered. (3) To approach the role of the NPY-immunoreactive neurons in cardiovascular-regulation center in the development of atherosclerosis. Methods (1) The animals was divided into A> B and C groups, the model of atherosclerosis was established. (2) HE staining (3) Immunohistochemical ABC method was used. (4) The Computer Image Analyser was applied to. Results (1)The atherosclerotic plaques were founded in the artery of the atherosclerotic rabbits. (2) The proliferation of the endothelial cells and the smooth muscle cells , the deposition of lipid, the stack of the foam cells and the atherosclertic plaques in the wall of artery were observed (3)The NPY-immunoreactive neurons were located in the frontal area of CG. In A group, the NPY-immunoreactive neurons in CG were fusiform, oval or multiangular, the nucleus and processes were obvious. The number of the NPY-immunoreactive neurons was 24.7 ±6.3, the average volume(v") of the NPY-immunoreactive neurons was 182.6 ± 45.3Mm3; In B group, the NPY-immunoreactive neurons in CG were oval or multiangular, the nucleus and processes were obvious. The number of the NPY-immunoreactive neurons was 39.3 ± 6.1, the v was 105.6 ?28.2Mm3 ; In C group , the NPY-immunoreactive neurons in CG were oval or multiangular, the nucleus and processes were obvious. The number of the NPY-immunoreactive neurons was 42.1± 7.7, the v" was 108.1 ±27.9um3. there were great difference between the A and B, Cgroups(P<0.01), but there were no significant difference between the B and C groups(p>005).(4) In A group, the NPY-immunoreactive neurons in ARC of hypothalamus were fusiform or multiangular, the nucleus and processes were obvious. The number of the NPY-immunoreactive neurons was 22.3 ± 6.1, the v was 382.3 ± 114.5Mffl3; In B group, the NPY-immunoreactive neurons in ARC of hypothalamus were oval or multiangular, the nucleus and processes were obvious. The number of the NPY-immunoreactive neurons was 38.6±7.5, the v" was 150.6 ± 43.2Mm3; In C group, the NPY-immunoreactive neurons in ARC of hypothalamus were fusiform or multiangular, the nucleus and processes were obvious. The number of the NPY-immunoreactive neurons was 36.9 ± 7.9, the v was 151.7±44.3Mm3; there were great difference between the A and B, C groups(P<0.01), but there were no significant difference between the B and C groups(p>0.05). (5) In A group, the NPY-immunoreactive neurons in RVL were spherical or oval, the nucleus and processes were not obvious. The number of the NPY-immunoreactive neurons was 35.2 ± 7.9, the v" was 102.3 ± 27.2Mm3 ; In B group, the NPY-immunoreactive neurons in RVL were spherical or oval, the nucleus and processes were not obvious. The number of the NPY-immunoreactive neurons was 48.2 ± 8.3, the v" was 210.4 ± 66.2Mm3; In C group, the NPY-immunoreactive neurons in RVL were spherical or oval, the nucleus and processes were not obvious. The number of the NPY-immunoreactive neurons was 45.1 ± 8.6, the v" was 205.9 ± 59.6Wn3; there were great difference between the A and B>C groups(P<0.01), but there were no significant difference between the B and C groups(p>0.05). Conclusions (1)There were great morphologic changes of the NPY-immunoreactive neurons in the cardiovascular-regulation center of atherosclerotic rabbits. (1)The NPY-immunoreactive neurons in the cardiovascular-regulation center might be correlated with the development of atherosclerosis. (3)The inhibitory role of VitE which played in atherosclerosis might not be correlated with the NPY-immunoreactive neurons in the cardiovascular-regulation centers.