Experimental Study On Tissue-engineered Bone Fabricated With β-Tricalcium Phosphate/Polyactic Acid, Coral Hydroxyapatite

Bone defect is one of the problems in the orthopaedics. The present treatments have allografts and biomaterial-filling. But they arenot ideal methods. Tissue-engineering has provided a new way to solve the problem,and has been one of hotspots recently.The loading segmental bone defect is very difficult to treat. Bone tissue-engineering of it involved biomaterias and fabricating of tissue-engeered bone. In this study B-TCP/PDLLA and CHAP were examined. [Objective]1.To inspect the characteristic of B-TCP/PDLLA and influence of B -TCP/PDLLA and CHAP on BMSc respectively in vitro.2.To fabricate tissue-engineered bone with B -TCP/PDLLA and BMSc,CHAP and BMSc respectively in vitro and observe the repair of goat tibia segmental bone defect with them in vivo. [Method]1. 3 -TCP/PDLLA and CHAP were dipped in 0.9% NaCL and examined its characteristic and biodegrade in vitro. BMSc of goat was cultured with B -TCP/PDLLA and CHAP respectively. The extending speed of the cells to the biomaterials,the prolferation and alkaline phosphatase activity were tested at 3,6,9 days in vitro.2.Eighteen goats were prepared with tibia segmental bone defects fixing with plate and divided into two groups. Contral group was repaired with the composite of 3 -TCP/PDLLA and BMSc. Experimental group was filled with the composite of CHAP and BMSc.The repairment was valued with X-ray,scanning electron microscope,histology at 4.8.12 weeks postoperative.[Results]1. β-TCP/PDLLA could biodegrade in vitro.BMSc survived at the surfaces of B -TCP/PDLLA and CHAP. BMSc cultrued with P -TCP/PDLLA and CHAP could proliferate and had ALP activity in vitro.2.The composite of CHAP and BMSc has repaired the goat tibia segmental bone defect,but the composite of P -TCP/PDLLA and BMSc has not. [Conclutions]1. β-TCP/PDLLA has good characteristic and biodegrade in vitro. There were no inhibition to cellular proliferation and ALP activity of BMSc when BMSc was cultured with P -TCP/PDLLA and CHAP respectively in vitro.2.The capability of bone forming of tissue-engineered bone fabricated with CHAP and BMSc was better than it of tissue-engineered bone fabricated with P -TCP/PDLLA and BMSc in vivo.