Experimental Studies On Culture Of Human Bone Marrow Stromal Cell In Vitro And Construction Of Tissue-Engineering Bone

Reconstructive, orthopedic, and plastic surgeons are continually faced with challenge of replacing bone lost by trauma, tumor or congenital abnormalities. Bone tissue engineering of autologous bone seems to be a promising method for repairing bone defects in clinical practice.Materials: In this study, human bone marrow stromal cells(HBMSC) were used as seed cells of bone tissue engineering. The biological characteristic of HBMSC cultured in vitro was studied. The biological effects of recombinant human bone morphogenetic protein-2 (rhBMP-2 ) and basic fibroblast growth factor( b-FGF)on HBMSC were also investigated. To test the feasibility of the compound artificial bone in vivo, using coral as scaffold , the HBMSC/coral composites were implanted subcutantously into athymic nude mice.Method: HBMSC obtained from human iliac crest was cultured in different medium. During culture, the growth and the biological characteristic of HBMSC were observed by cell morphological survey, cell growth curve, scanning electron microscope(SEM), alkaline phosphatase(ALP) stains and Von Kossa stains. The biological effects of rhBMP-2 and b-FGF on HBMSC were measured with methylthiazoltetrazolium (MTT) method and ALP measurement kit. The effectiveness of new bone formation was evaluated by means of gross, histological observation and SEM examination.Result: HBMSC can be cultured in vitro and induced into osteoblast. rhBMP-2 and b-FGF can promote the proliferation and differentiation into osteoblast of HBMSC. In immunodeficiency animal subcutantous tissue, the ectopic osteogenesis of HBMSC/coral composite was obvious, and the new bone formation increased and became more mature in pace with time.Conclusion: HBMSC can be used as seeded cells in bone tissue engineering, made with only a small amount of aspired marrow from patient with little host morbidity. rhBMP-2 and b-FGF can promote the proliferation and differentiation of BMSC. The HBMSC/coral composite can form bone tissue in vivo, showing a promising prospect for clinical practice.