| Infantile capillary hemangioma (ICH) is one of the most common benign inalacoid tumors of infancy. they could bring about all kings of damage because they occur in every place of one body with a high morbidity and rapidly proliferation. Therefore , it is urgent to cure them timely and effectively .Pingyangmycin(PYM) is a kind of popular drug which is used for hemangiomas' treatment. But the traditional way of PYM being injected into tumor has more side effects, so PYM was made into emulsion and smeared the surfaces of the tumors evenly in order to reduce the extra damage. Many documents suggest that PYM can induce apoptosis in malignant tumor in order to cure them .Furthermore , the tendency that capillary hemangiomas gradually wither away with themselves in their course of disease is evidence . So that , it is necessary to study whether PYM emulsion effectively promote apoptosis in hemangiomas in order that the tumors could die more rapidly. Objective : To insight whether PYM emulsion induce apoptosis more rapidly in proliferating capillaryhemangiomas and further inquiry the mechanism at molecular level so as to find the optimal method for hemangiomas' treatment. Method: The specimens were all the epizoic tumors which were obtained from the children whose ages were all lower than 6 months . They were divided into 2 groups , A and B, every group counts 15. Group A was used as control , whereas group B were experimental group. PYM was made into emulsion and smeared the surfaces of the tumors. This course lasted about 7 days, 3 times every day . The specimens were resected by operation on day 7,then made into pathological slices and electron microscope slices . Furthermore , the apoptotic cells in group A and B were tested by molecular biology method (TUNEL test ). Results: 1. About 2/3 of group B became deep red and their surfaces began to shrink slightly after having been treated by PYM emulsion . Those of group A kept the same as before . 2. Light micrographs showed that the endothelial cells of group A proliferated actively and tended to gather together like a ball, the capillary lumens were closed up and the little vascular scattered fasciculatly or foliolarly . However , the endothelial cells of group B became less and ranked bunchily and the lumens was easily seen . There were adipose tissue and fibrous tissue between the cells .3. The results of electron micrographs : the cells of group A proliferated actively and closed together whoseappearances were normal and chromatin distributed evenly . There were abundant organelles such as mitochondria , endoplasmicreticulum and golgi complex in the cells . The apoptotic cells were few while the mast cells was easily seen that gathered beside the capillary lumens . On the contrary ,There were much apoptotic phenomena in group B: The external forms of the cells became shrinkage , the chromatin assembled near the nuclear membrane and formed crescent body, the cell-cell junctions disappeared and cytoplasm became condense , all kinds of organcelles condensed and closed each other . Furthermore , pycnosis and caryoclasis were seen in the test . In addition ,we also found the apoptotic-initating signal―chromatin phalangeal process. The mast cells became less than that of group A. 4. The results of TUNEL test were agree with those of electron micrographs . There was a small amount of yellow -brown apoptotic cells scattering among the blue normal cells in group A.The cells of group B showed the higher apoptotic rate. Part of apoptotic cells lied in the surfaces of the lumens .The more close to the lumens , the more high rate of apoptosis. The results of statistics showed that the apoptotic index (AI) of group A was 9.693±4.948 and that of group B was 39.373±15.927, there was extraodinary significant diffrence between A and B (P<0.01). Condusion : ICH present a tendency of spantaneous apoptosis . As a way of curing hemangiomas ,PYMemulsion can effectively accelerate apoptosis majorly in their endothelial cells...
|
PDF Full Text Request