| Cerebrovascular and cardiovascular diseases are the most harmful to public health. The main pathogenic factors, especially the high level of blood cholesterol, is the disorder of lipid metabolism. How to regulate the blood lipid effectively is a hotspot on nutriologyresearch.Objective: To investigate the regulation of tea polysaccharides on lipid and its mechanismin hyperlipidemic rats.Methods: Male SD strains rats (150-170g) were randomly divided into one normal group fed normal diet(10 rats) and experimental groups(36 rats) fed high-lipid-diet according to the blood cholesterol and the weight. After the experimental groups were fed high-lipid-diet for four weeks and the models were prepared successfully(27 rats chosen out), they were redivided into three groups: the control group, the low dosage group (TPSI), and the high dosage group (TPSII)(9 rats per group). The normal group was kept on feeding with normal diet while the other three groups with high-lipid-diet. The groups of TPSI and TPSI1 were administrated with TPS at the dosages of 100mg/kg and 200mg/kg, respectively. According to the two dosages, two concentrations of TPS of 3ml per day for one rat were prepared while the normal and the control group with the same volume of 0.9%NS. The experiment lasted two months. During the course, the fast blood cholesterol (TC), low-densitycholesterol (LDL-C), high-density cholesterol (HDL-C), were tested at 20th day (the early stage), 40th day (the mid stage), and 60th day (the late stage).And at the late stage,the activity of the fast blood LCAT, LPL, HL were also determined and meanwhile HDL-C, HDL2-C, HDL3-C were tested using the same share of the plasma.At this time,the groups with high-lipid-diet changed with normal diet.After one week, by the end of the test, the rats were killed at 23 to 1 o'clock and their livers were quickly removed. The activity of liver HMG CoA reductase and the degrees of the fat accumulations on livers were studied. Statistical calculations were performed with commercial package of SAS 8.0 and SPSS V10.0. Values were expressed as means±S.Results:1. The effects on blood lipids in hyperlipidemic rats: (l).The blood TC level of TPSI group at the early stage, mid stage and late stage were decreased 8.6%, 7.0%, 16.0% respectively, but there was no difference compared with those of before administration; The TC level of TPSII group was decreased 11.9% significantly at the late stage compared with that of before administration. After given TPS for two months, compared with the control group, the TC level of two experimental groups were reduced 21.0% and 20.1% significantly. However, there was no difference between the two experimental groups (P>0.05). (2).TPS played an important role on increasing the concentration of blood HDL-C. The value of HDL-C in TPSI group were increased 12.9% and 23.0% at the mid stage and late stage compared to those of before administration, thereinto the value at the late stage increased significantly; while the TPSII group were elevated 23.9% and 22.5% markedly at the same time. At the end of the experiment, the level of HDL-C in TPSI and TPSII groups were increased 18.7% and 21.6% significantly compared with the control group. (3). TPS has no significant effect on the concentration of the fast blood LDL-C.2. The effects on the anti-arteriosclerosis factors (HDL-C/TC, HDL-C/LDL-C): The two ratios could be elevated greatly. The ratios of HDL-C to TC (HDL-C/TC) in TPSI and TPSII groups were increased 46.7% and 39.1% compared to those of before administration,while increased 57.1% and 52.4% compared to the control group (P0.05). Although TPS couldn't increase the ratio of HDL-C to LDL-C (HDL-C/LDL-C) compared to that of before administration, its value in the two experimental groups, compared to the control group, was respectively elevated 22.1% and 17.7% markedly (PO.05).3. The effects on the activities of LCAT, LPL, HL and HMG CoA reductase: (1). LCAT: The activity of LCAT in control group was lowered greatly th... |
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