Regulative Effect Of Extract Of Lindley Eupatorium Herb On Blood Lipids And Mechanism In Experimental Hyperlipidemia Models

BACKGROUND AND OBJECTIVESHyperlipidemia has been clinically common and frequently occurring disease recently. It results that the heart and cerebrovascular disease, like coronary heart disease, atherosclerosis and cerebral thrombosis, also have been serious disease that threatening human health. Recently, we are used to some kinds of chemical medicine to cure these diseases, however, there are too many adverse reactions to take these kinds of medicine in a long term. Therefore, it is a clinically significant thing that we try to find out the traditional drug, both less adverse reaction and the effective pharmacology. Lindley eupatorium herb(LEH) is the drying aerial part of Eupatorium lindleyanum DC.var.trifoliatum Makino, and it can clean lung-heat, stop cough, dissolve sputum, lower hypertension, and so on. In the chronic toxicity trial of the extract of LEH, we occasionally find that the level of total cholesterol (TC) and triglyceride (TG) in blood plasma of animals in LEH group is lower than other groups. Recently, there has not been a research report that reports the action. This new pharmacology action of the single traditional drug is valuable, so we decide to observe the regulative effect of LEH on blood lipids and mechanism.METHODSThe first part observed the effect of LEH on blood lipids in two different hyperlipidemia models: mice injected with yolk and rats drenched with high fat suspension. Mice were randomly divided into four groups: normal control group, high-fatty model group, positive control group, LEH high dose and low dose group. Rats were randomly divided into six groups: control group, high-fatty model group, positive control group, LEH high dose, middle dose and low dose group. All mice except those in normal contro1 group were injected intraperitoneally with 75% yolk after treated with the corresponding drugs 30 days,and all rats except those in normal contro1 group were drenched with high fat suspension and meanwhile being treated with the corresponding drugs for 60 days. The influence of LEH on blood lipids in mice and rats were observed. Meanwhile, we analyzed the hepatic steatosis situation of rats.The second part had established hyperlipidemia model in rats, drenched with high fat suspension (It is same with part one). After 60 days, we observed the level of free fatty acid(FFA), lipoprotein lipase(LPL), hepatic lipase(HL), lecithin cholesterol acyl transferase (LCAT). Meanwhile, we analyzed the changes of 1ow density lipoprotein receptor (LDLR) gene expression in liver by RT-PCR in order to discuss the mechanism of LEH regulating the level of blood lipids.RESULTS1. LEH could significantly decrease the level of TC,TG and LDL-C, and markedly increase the level of HDL-C in two different hyperlipidemia models: mice injected with yolk and rats drenched with high fat suspension.2. The situations of hepatic steatosis in LEH groups, especially the high dose group, were fairly lighter than those in high-fatty model group.3. LEH could significantly decrease the level of FFA in the experimental hyperlipidemia rats. The activities of LPL, HL and LCAT in blood plasma of rats in LEH groups, were significantly much higher than those in high-fatty model group. Furthermore, it can markedly enhance LDLR gene expression in liver.CONCLUSIONLEH could regulate the level of blood lipids, promote the transfer of cholesterol from peripheral tissue cell to liver cell, activate the activity of lipid metabolism enzyme, and enhance the expression of LDLR mRNA to modulate the lipid metabolic disorders in hyperlipidemia rats to avoiding the happen of atherosclerosis (AS).