The Regulation Of Lotus Leaf Flavones On Blood Lipids And Antioxidation In Rats

Cerebrovascular and cardiovascular diseases have been the chief diseases threatened people's health. The main pathogenic factor of the Cerebrovascular and cardiovascular diseases is the disorder of lipid metabolism. How to regulate the blood lipid effectively and antioxidation has been a hotspot on nutriology research in nowadays.Objective: To investigate the regulation of lotus leaf flavones on lipid and antioxidation of lotus leaf flavones in rats.Methods: Sixty male rats were randomly divided into six groups according to the blood cholesterol and the weight, each group had ten rats. The groups were divided as normal group, hyperlipidemic control group, and high dosage of lotus leaf flavones(LLF) group, low dosage of lotus leaf flavones group, lotus leaf flavones + propolis group, and propolis group. The normal group was fed on normal food, while other group's feed were high-lipid-fodder which was constituted of 10 percentage lard and 10 percentage yolk powder and 80 percentage normal feed. The experiment lasted sixty days. In experiment, the normal group and hyperlipidemic control group were administrated with 0.9%NS;the high dosage of lotus leaf flavones group was administrated with the lotus leaf flavones at the dosage of 60mg/kg;the low dosage of LLF group was administrated with LLF at the dosage of 30mg/kg;the LLF and propolis group was administrated with LLF and propolis at the dosage of 30mg/kg and the propolis group was administrated with propolis at the dosage of 60mg/kg. During the course, the rats are forbidden to eat for 12h at the 0, 20, 40, 60 day, and tested the TC, TG, and HDL-C. At the sixtieth day, the LPL, HL, SOD, GSH-PX, MDA of rats was tested after the rats had been forbidden to eat for 12hours. Then, the high-lipid-feed was changed into normal-feed. The rats were killed at the time 23:00 to l:00(the next day) after a week, and the livers should be taken at once. The activity of liver HMGCOA reductase and the degrees of the fat accumulations on livers were studied. Statistical calculations were performed with commercial package of SPSS VI 2.0.Values was expressed as means ±s.Results:l.The effects on blood lipids in experimental rats: (1) The effects on experimental rats' weight: At the beginning of the experiment, there was not significantly difference among the rats. At the early stage of the experiment, the average weight had a significantly raise compared with the former, and the difference was significant. (P<0.05). Among the groups, the normal and hyperlipidemic control group had significantly difference. (P<0.05). At the middle stage of the experiment, the average weight was heavier than the former stage again. Besides the difference between the normal group and the hyperlipidemic control group which was existing at the first stage, the difference between hyperlipidemic control group and the high dosage of LLF has been significantly. At the last stage of the group, the average weight has a raise again, and thecomparison result is as same as the middle stage. At these stages, the four experimental group had no significantly difference. (2) The effect on the jats' TC: at the beginning of the experiment, the groups had no difference. At the early stage, only the hyperlipidemic control group had a significantly raise compared to its primacy data. The difference was significantly. (P<0.05). At the middle stage of the experiment, there was still the hyperlipidemic control group raised. The difference was significantly. On the comparison of the control group and experimental groups, there were significantly differences. (PO.05). At the last stage, there was still only the difference between hyperlipidemic control group and its former was significantly. Among the groups, the difference between hyperlipidemic control group and the experimental groups (the high dosage of LLF group, the low dosage of LLF group, the LLF and propolis group and the propolis group) were significantly. There was no difference among the experimental groups. (3) The effect on rats' TG: At the beginning of the experiment, there was no difference among the groups. At the early stage, the normal group and hyperlipidemic control group' had risen compared to its primacy data. Among the groups, the hyperlipidemic control group was significantly different from the other experimental group.(P<0.05). At the middle stage, the TC level of the hyperlipidemic control group and the propolis group had risen significantly. Among the groups, the hyperlipidemic control group was significantly different from experimental group. (P<0.0 5). At the last stage, the result was the same as the middle of the stage. (4) The effect on the rats' HDL-C: At the beginning of the experiment, there was no difference among the groups. At the early stage, the normal group was the same as its primacy level, the HDL-C level of the hyperlipidemic control group was reduced, and the experimental groups all had risen to a certain degree. But only the hyperlipidemic control group has asignificantly reduce. Among the groups, the hyperlipidemic control group had a significant different from the high dosage of LLF group and the low dosage of the LLF group.(P<0.05). At the middle stage, the HDL-C level of the hyperlipidemic control group had reduced significantly. Among the groups, the differences between the control group and the high dosage of LLF group, the control group and the low dosage of LLF group, the control group and the propolis group were significant.(P<0.05). At the last stage, there was still only the hyperlipidemic control group reduced significantly compared to the primacy level. Among the groups, the hyperlipidemic control group had a significant difference from the experimental group. There were no significant different among the experimental groups.2. the effect on the rats' HL,LPL,HMG CoA: (1) the effect on the activity of HL, the activity of the hyperlipidemic control group was greatly lower than that of normal group and experimental groups.. Among the experimental groups, the LLF + propolis group was raised most greatly, followed by high dosage of LLF group. The LLF + propolis group had a significant different from the low dosage of LLF group and propolis group. The high dosage of LLF group had a significant different from the low dosage of LLF group and propolis group. No significant difference was observed between the high dosage of LLF and LLF+ propolis group. (2) The effect on the activity of LPL: the activity of the hyperlipidemic control group was markedly lowered than that of normal group and experimental groups. Among the experimental groups, the high dosage of LLF group had the most significant raise, followed by the LLF +propolis group, and the propolis group was raised lest significant. Significant difference was observed between the high dosage of LLF group and propolis group, the LLF + propolis group and propolis group. (3) the effect on the HMG-GoA: the activity of the hyperlipidemiccontrol group was significant higher than that of normal group and experimental groups (P<0.05) . Among the four interference groups, the LLF +propolis group reduced most significantly, followed by the high dosage of LLF group and the low dosage of LLF group, and the propolis group reduced the lest significant. Significant difference was observed between the the LLF +propolis group and propolis group, the LLF +propolis group and high dosage of LLF group, the LLF +propolis group and low dosage of LLF group.3.The effect on the SOD,MDA,GSH-Px : (1) The effect on experimental rats'SOD: the activity of hyperlipidemic control group's SOD was observably lower than the experimental groups (P<0.05) .(2) The effect on experimental rats'MDA: the hyperlipidemic control group'MDA was significant higher than normal group and the four experimental groups(P<0.05). No significant difference was observed among the interference groups. (3) the effect on experimental rats'GSH-Px: the activity of the hyperlipidemic control group was observably lower than the normal group and the experimental groups (P<0.05) . Among the experimental groups, the activity of the high dosage of LLF group was the highest one while the low dosage of LLF group was the lowest one. The difference between these two groups was significant while there was no difference among the other groups.Conclusion:1. The lotus leaf flavones has significant effect on lowering rats' weight,TC,TQHMG CoA, heightening HDL-C,HL,LPL.2. The lotus leaf flavones has significant effect on antioxidation. The lotus leaf flavones has significant effect on heightening SOD,GSH-Px,lowering MDA.