| Ischemic cerebrovascular disease (ICVD) is seriously dangerous to the health of human. It has high mobility, big incidence of a handicap, and bad prognosis. Recently, it has been recognized that rehabilitative training can promote the renew of sense and motor function of ICVD. But it's neurobiological base is not clear, To study the mechanism how the rehabilitative training promote the renew of nerve function, We established a model of photochemically induced rat's cerebral thrombus and gave it rehabilitative training, then observe the changes in sense, motor and memory function , histomorphology and GAP-43 .PCNA in central nerve system at different time. The experiment consists of four parts.1. Establishment of photochemically induced rat's cerebral thrombusThe CPD4 of different concentration was injected into rats and then He-Ne laser was irradiated on the left contex areas of the forelimbs and hindlimbs ,We observed the changes in neurobehavior pathomorphology and the sizes of infarction 24 hours later. Results: Cerebral infarctions were induced by laser irradiation plus 40mg*kg"' or 50mg籯g~'CPD4 ,The infarction sizes were stable ,the changes in neurobehavior and pathomorphology were obvious. The infarction model induced by CPD4 showed a good stability, excellent repetition and high rate of success.2. The effect of rehabilitative training on the recovery of therats' neural and memory function after cerebral infarctionSD rats were given cerebral infarction and divided into 3 groups at random 24 hours laterrehabilitation group which were given balancing, grasping, rotating and walking exercises everyday, braking group which were fixed in mesh cages and free group which were feeded in normal cages, The neural functions of the three groups were evaluated at 24h,lw,2w,3w and 4w after infarction.The abilities to study and remember were compared at different time: before infarction, 2w and 4w after infarction. Results: Rehabilitation group showed better function than the other two groups , Conclusion: Rehabilitative training can promote the recovery of the cerebral infarcted rats' motor functions' As well as the ability to study and remember.3. Change of histomorphology of the cerebral infarcted rats1 after rehabilitative trainingSD rats were given cerebral infarction induced by photoeltomical injury and were divided into 3 groups at random, and detect the volume of infarction tissue , the change of morphology microscope and the blood vessel network density at 24h, lw,2w,3w and 4w later. Results : The volume of brain infarction was the largest at 24h and obviously reduced at lw,especially in the rehabilitation groups(P<0.05), Obvious necrosis inside brain infarct focal was seen at 24h .It was shown in the rehabilitation groups that glia cells had hyperplasia in edge of brain infarction and a lot of blood vessel sprout into infarction area at Iw. The granulation tissue and bracket of blood vessel formed in the brain infarction focal at 4w , The density of the blood vessel outside the infarction(lw) underside the infarction (l-2w)and contralateral corresponding area (Iw) were obviously higher in rehabilitation group than in braking group (P<0.05), Rehabilitative training can reduce thevolume of infarction early , increase them promote the collateral circulation , improve ischemia and regeneration and repairment of brain tissue .4. Expression of GAP-43 and PCNA in CAN of brain infarcted rats after rehabilitative trainingThe changes of GAP-43 ,PCNA in brain were immunohistochemically studied at 24 h> 3d> 7d^ 14d^ 21 d> 28 d after infarction. Results : GAP-43 stained neurons were detected around the infarcted area at 24 hx 3 d^ 7 d^ 14 d and were diminishing at 2K 28. The increase was obvious in rehabilitation groups than braking group at Id* 14d (PO.05). especially in callose. Brown and yellow PCNA cells were seen around the infarcted area at 7> 14* 21 > 28d,most of which are astrocytes, vasal endothelium cells and macrophages.Rehabilitation training group reacted more obviou...
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