The Expression Of VEGF, BFGF And P16 In Osteosarcoma And Their Relationships

Study Design. The Immunohistochemical method was used to detect the expressions of VEGF(vascular endothelial growth factor), bFGF (basic fibroblast growth factor) and p16 on osteosarcoma sections respectively, and the results were processed with Spearman rank correlation test.Objectives. The geol of this study was to investigate the expression rate of VEGF, bFGF and p16 in osteosarcomas, and to study the relationships between any two of the three factors.Summary of background data. VEGF and bFGF are two most important growth facl n-s relating to angiogenesis and cellular proliferation. Recently, they were found to have relations to tumorigenes is. Both VEGF and bFGF were found high expression rate in many kinds of malignant tumors, such as bladder cancer, lung cancer, gastric cancer , brain cancer and so on, but the relationship ?between VEGF and bFGF had not been clearly reported. P16 is another important factor responsible for inhibition of tumorigenesis, but its expression rate in a variety kinds of ma 1ignant tumors remain controversial. Moreover the correlation between p16 and VEGF, p16 and bFGF in cancer tissues had not been reported yet. Methods. 13 osteosarcoma histopathologic specimens were selected at random, with each cut into 3 section preparations. All section preparations wee divided into 3 identical groups, with each group containing each one of the 13 section preparations. The 3 groups of sections were immunohistochemically detected with VEGF, bFGF and p16 kits respectively. Spearman's rank correlation was used to analyse the results.Results. The expression rates of VEGF, bFGF and p16 were 76. 9%(10/13), 61. 5%(8/13) and 38. 5%(5/13) respectively. The correlation coefficient between VEGF and bFGF had high statistical significance (rs=0. 803, p<0. 01). and those between p16 and VEGF, p16 and bFGF were not statistically sign.icant (rs=0. 423 and 0. 330, p>0. 05). Conclusions. The .-xpressions of VEGF and bFGF in osteosarcoma were closely correlated, while that of p16 was irrelevant to VEGF and bFGF. The expression rate of p16 in osteosarcoma was relatively lower than normal tissues, which demonstrating the deactivation of p16 is partly responsible for tumorigenesis.