| Chronic Atrophic Gastritis (CAG) is a common disease in clinic. Because of its positive correlation with morbidity of gastric carcinoma, it was considered as a precancerous condition by World Health Organization (WHO). For this reason, to prevent and cure CAG plays a very important role in preventing gastric carcinoma. In this study, we reestablished an animal model of rats with CAG in accordance with the previous experience of combined administration of 60% ethanol, 20mmol/L sodium deoxycholate and 0.1% ammonia water. In one group, sucralfate and isinglass were used as preventive therapy while we were establishing CAG rat model. In the other group, sucralfate and isinglass were used to treat rats after establishment of CAG rat model. To explore a new method of preventing and treating chronic atrophic gastritis is the ultimate aim of this study.Materials and Methods80 healthy, sexual mature, qualified female SD rats were tested, weighting 200+lOg, provided by Zhejiang Medical Institute. All the rats were randomly divided into 8 groups. The rats in Normal Control Group were raised in a routine way for 32 weeks. The rats in Model Control Group were administered with 60% ethanol, 20mmol/L sodium deoxycholate and 0.1% ammonia water for 24 weeks. Then they were fed in a routine way for 8 more weeks. Three groups of rats under prevention test were administered by isinglass 160mg/100g/d, 80mg/100/dand sucralfate 160mg/100g/d respectively in each morning for 24 weeks. Three groups of rats in reverse test were continuously administered by isinglass 160mg/100g/d, 80mg/100g/d and sucralfate 160mg/100g/d respectively in each morning for 8 more weeks after the CAG model was established. Then these rats were anaesthetized by 1% thiopental sodium in foodless condition. 5ml/per blood were taken from carotid through catheter. All rats were executed by dislocation of cervical vertebra and their stomachs were removed and observed the gastric plica,elasticity and luster. The gastric streak-like samples for histological examination were cut along their lesser curvature and greater curvature respectively. Then they were immediately immersed in 10% buffered formalin and embedded in the paraffin. Paraffin sections were cut, mounted on glass slides, and stained with hematoxylin and eosin (HE) to observe a series of indices as follow: (1) Inflammation grade of gastric antrum and body basing on semi-quantity, (2) Ratios of the thickness of gastric mucosal gland and muscularis mucosa(Ll/L2), (3) The number of gastric glands inl-mm lengths of mucosal layer in longitudinal sectionsResults and DiscussionThe experimental results showed the rats in isinglass prevention groups and reverse groups were brisker and heir hairs were whiter and more lustrous than those in CAG model. And the gastric plica, elasticity, luster of rats in the former groups were better than those in letter groups. There were more mucus adhered to the gastric mucosal surface of the rats in the former group than in the latter groups, in which there were obsolete petechia, spot- like or speckle-like erosions in the gastric mucosal surface.The experimental results showed the CAG model was successfully established by administration of 60% ethanol, 20mmol/L sodium deoxycholate and 0.1% ammonia water. HE slides revealed: mucosal glands reduced and arrayed disorderly. Mucosal muscle layer became thick. Plenty of Inflammatory cells immersed in gastric mucosa, partly congregated. No intestinal metaplasia and atypical hyperplasia were found.Pathological patterns of isinglass prevention groups were similar to that of normal control group. However pathological patterns of isinglass reverse group were much better than that of model control group, even close to that of normal control group. HE slides revealed: mucosal glands arrayed closely and regularly. Only a few of inflammatory cells were found.After preventive treatment with isinglass, the inflammation grade, the means of L1/L2, 1-mm lengths of mucosal layer in longitudinal sections of gastric ant...
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