Study On Fingerprints And Purity Identification Of Spring Rapeseed (brassica Napus L.)

In lower altitude areas of Qinghai Province where mainly planting Brassica napus, there are seven varieties that contains six hybrids, one conventional variety. identificate Hybrids authenticity and purity rapidly that it is important for fight against counterfeiting and protected the interests of breeders and farmers has grateful significance. In the past mainly testing purity use morphological in the field and that can offer limited detection targets, morphological identification way can not adapt for purity analysis more and more. Molecular markers have been developed in recent years, this way reflection the differences of genomic directly that has been applied to constructed agriculture crops and varieties fingerprinting and identification widely, that is an effective mean for hybrids authenticity identification. In this study, application of SSR molecular markers constructed 14 Cultivars spring Brassica napus fingerprints of Qinghai and study on Qingza lines hybrids rapid purity identification way, as the following results:(1) 13 pairs polymorphic primers screened from 140 pairs SSR primers that using Construction fingerprints showed that the primer combinations P013 + P052 or P086 + P027 + P021 amplification bands can be completely distinguished14 materials that contains 4 hybrids and its parents, Huaxie No1 hybrids,Hufeng010 hybrids and Qingyou No14. Those fingerprints can be used for parent materials and hybrids authenticity identification.(2) Selected SSR primers have co-dominant bands that adapt to varieties purities identification. Qingza No.2 hybrid (P019,P027), QingzaNo.3 hybrid (P013,P027,P052),Qingza No. 4 hybrid(P027,P052,P086) ,Qingza No.5 hybrid (P030,P085).(3) Using above primers identified the hybrids purities that production of 2008, by field cultivation methods identified those hybrids Purity meanwhile. Laboratory testing hybrid purity: Qingza 2 Purity:83.41%(P027),83.90%(P019);Qingza No. 3 purity:87.02%(P027),86.54%(P052),86.06%(P013);Qingza No. 4 purity:70.67%(P027),69.23%(P052),68.75%(P086);Qingza No. 5 purity:85.37%(P030),82.93%(P085).Respectively, the field identification purities were 88.29%, 91.35%, 72.12%, 87.80%;through comparison lab. and field purities identification results, indicated that SSR markers tested purities all lower than field purities, that because of field planting identification purity according for saxes, and SSR marker not only distinct sterile from hybrids, but also districted more Restorer, and other rape plants in the field.(4) 2009 winter in Yunnan tested materials that from Qingza No. 2 field tested hybrids, lab. tested sterile bands and restorer bands plants that crossed with true sterile, and tested self crossed seeds that from those plants self cross. Indicated that those hybrids from lab. tested sterile bands plants crossed with true sterile were all true sterile, self cross seed were all maintainers, and hybrids from lab. tested restorer bands plants crossed with true sterile were all true hybrids, self cross seed were all restorer. Indicated that SSR marker testing hybrid purity was correct than field plant testing.