Identification Seed Purity Of "Shaanyou803" In Brassica Napus L. And Molecular Markers Of One Gene Underlying Chlorophyll-deficit Trait In B. Juncea

Utilization of heterosis is one of the effective means to improve the yield and quality inrapeseed. The area of Huanghuai River is one of the important areas of rapeseed production inChina, so the present investigation aimed to breed a new hybrid rapeseed (B. napus) varietywith excellent agronomic characters, which was suitable for planting in the reach ofHuanghuai River and the similar ecological planting regions in China. The fast and accuratepurity identification of rapeseed hybrid is important basis work for safe promotion,high-efficience of production. Shaanyou803(HZ03), a new hybrid rapeseed variety withdouble quality(low erucic and low glucosinolate content), was developed using cytoplasmicmale sterile line ZS09A and restorer line SH11in2008by College of Agronomy, NorthwestA&F University, which was approved by China Crop Variety Approval Committee in2012,called Shaanyou803(HZ03)(Guoshenyou2012011).Some factors directly or indirectly affect the synthesis or degradation of chlorophyll,alter the chlorophyll content of leaves, and finally lead to the leaves’ color mutation. Thesekind of mutants have significant variation compared with the normal colorleave.Chlorophyll-deficient mutants are ideal materials for studying the inheritance of leafcolor, chloroplast ultra structure and development, chlorophyll biosynthesis, photosyntheticphysiology, pigment-protein complexes, location of leaf color mutant gene, identification ofgene function, the nucleocytoplasmic gene interactions, and provide new elite germplasmresources for breeding of high light efficiency, and also serve as marker traits at seedling stagefor identification of the purity of the hybrid seeds.This paper reports the breeding process of Shaanyou803; Using hybrid rapeseedShaanyou803, and its both parents as the materials, we established a SSR system to identifythe purity of Shaanyou803. By using the NIL (near-isogenic line) derived from the progenyof the cross between L638-y and2598as the material and AFLP with bulk segregant analysisstrategy, we aimed to locate a leaf color mutant gene gr1. The main results obtained are asfollows:1）Average yield of Shaanyou803in22winter rapeseed regional trials was224.56kg／667m2，which was increased by7.63%compared with the control Qinyou No.7from2010to 2012．And its average yield in six regional production trials was200.652kg／667m2，whichwas increased by4.92%compared with the control from2011to2012．Its oil content was41.32%, erucic acid content was0.05％，and glucosinolate content in meal was24.22μmol/g.Shaanyou803showed strong heterosis，early/intermediate maturity，cold and droughtresistance. It was suitable for extending in the reach of Huanghuai River and the similarecological planting regions in China.2）Among120pairs of SSR primers tested, two primers (BRMS-042, CB10026) werescreened out which could distinguish the male sterile line ZS09A and restorer line SH11ofShaanyou803. Amplification results of the SSR primers and fertility investigation in field atflowering stage are highly consistence with each other, the result is more reliable bycombining the both methods for identifying Shaanyou803purity.3）Twenty primers selected from120pairs of SSR primers were used to PCR analysisShaanyou803and the other33hybrids, which could distinguish Shaanyou803from other33hybrids successfully.Therefore, these20pairs of SSR primers can be used as characteristicprimers for construction fingerprinting of Shaanyou803.4）The NIL (near-isogenic line) derived from the progeny of the cross between L638-yand2598were used as the materials, two pairs of AFLP primers EC14TG4（EC-CA/TG-TC）and EC15TG3（EC-CG/TG-TG）were screened out from1200AFLP primers which couldamplify polymorphic bands.