| Vaccinium bracteatum Thunb. is regarded as a quite useful tree, because of the multi-value including health function, medical and ornamental uses. The distribution of Vaccinium bracteatum Thunb. was investigated in this paper. One hundred plants belonging to 20 provenances were collected and conserved. And on this base, nutrients dynamic changes among different provenances, softwood cutting propagation technology and cutting rooting anatomical observation were studied. The main results are as follows:1. Vaccinium bracteatum Thunb. was distributed on hills and mountains above 400-1400 sea level in south of Yangtze river. Vaccinium bracteatum Thunb. was a kind of plant, which preferred sunshine and acid soil environment, and it had capacity of tolerance to leanness. The major families consisting of Vaccinium bracteatum community were Fagaceae, Lauraceae, Fabaceae, Rosaceae, Theaceae and Verbenaceae.2. The dynamic changes of the main active substance (total flavonoids) and general nutrients (solubility proteins,amino acid and soluble sugar) contents in the leaves of different Vaccinium bracteatum provenances were studied in the paper. The results showed the trend that the content was the highest levels during May and June, gradually decreased over time, and went another slow upward to October. Comprehensive analysis of the trends of nutrients, leaves in Vaccinium bracteatum provenances should be picked during May and June or in October by the initial determination.Trend surface analysis of geographic variations was made with longitude and latitude being independent variable, the average content of nutrients being induced variable. The content of amino acid showed a center (crossing of 113°E and 26°N)-round variation, with the decrease from the center to the round. The variation of total soluble sugar content was also affected by latitude and longitude, and it showed the trend that it decreased from the southeast and northwest to the middle. However, there was no significant trend in the variation of total flavonoids and solubility proteins.Regarding nutrients content in June among Vaccinium bracteatum provenances as index, six superior provenances were preliminary selected for health purpose, which were respectively Jinhua in Zhejiang, Miluo in Hunan, Nanjing in Jiangsu, Yixing in Jiangsu, Guangde in Anhui.3. Wild Vaccinium bracteatum semi-lignified cutting propagation technology was studied in this paper. The result showed: IBA could significantly improve rooting rate, while ABT had the optimal rooting effect; the mixture substrate (peat soil 1: perlite 1) was the better substrate, with significant difference to others. It had the better rooting effect for cutting got from the dormant branches in spring.4. Anatomical study on rooting of Vaccinium bracteatum Thunb. cutting has been carried out by the paraffin section method. The observation result shows that the adventitious root primordium of Vaccinium bracteatum cutting belonged to the type of induced root primordium. The adventitious root primordium originates from proliferation of parenchyma cells in bud gap or the cross region of vascular cambium and pith rays. The difficulty of cutting rooting was closely related to the stem anatomical structure. Most of the base of the cuttings had produced callus, and callus played a role on transporting water and nutrients. |
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