An Application Research Of DNA Fingerprint In Identification Of Individuals And Traceability Of Pig

A suitable food tracing technology is important to the food traceability; more relevant departments and consumers pay close attention to it. Base on the DNA fingerprint technique, this research is focused on verifying the technical feasibility for identification of individuals and traceability in pig by microsatellite DNA markers, it is value to trace every piece of meat from plate to farm or track meat production from farm to plate.Relative research can use for reference, getting a pair of PCR primer through primer design sofrware for rapid detection of mitochondrial DNA of fresh porcine,bovine,goat and sheep meat, the amplification products showed that mitochondrial species-specific fragment of porcine meat was differ from others. Using this method we can identify the fresh meat species quickly. 25 pg porcine meat DNA can be detected. This method can be supplied to detection step of identification of individuals and traceability in pig.Base on the PAGE technique and PCR technique, the polymorphisms of fourteen microsatellite markers were analyzed in this study, and the result shows that it is possible to identify thirty pigs'individuals, which are the offspring of three parents of three different foreign breeds(Yorkshire, Landrace and Duloc). We can identify twenty pigs'individuals of two parents using seven microsatellite markers, ten pigs'individuals of another parent using fourteen microsatellite markers. In another study on traceability of fifty pigs from meat to blood use the sixteen microsatellite markers, it shows that the information of microsatellite loci are one-one correspondence, so it is feasible to trace the pigs from meat to blood.Ninety microsatellite markers are screened from NCBI data base basing on foreign research, which concluded that the effective population size and the mutation rate determine microsatellite variability. An optimum proposal is choosed: using Touchdown-PCR technique and 15% glycerol in the PCR reaction. It can decrease the non-specific amplification products. Forty two microsatellite markers are screened for identification of individuals of Bama miniature pig basing on this proposal. The result shows that it is possible to identify fifty seven pigs'individuals of eighty pigs using three microsatellite markers. If adding two markers, the result will be better: it is possible to identify sixty eight pigs'individuals of eighty pigs using three microsatellite markers.