The Anesthetic Effectiveness Of Electro-acupuncture Anesthesia Combined With Xylidinothiazoline And Its Effect On Some Physiological Functions In Goats

Acupuncture has operational advantages. Acupuncture also has less physical interference, so that animals recovered quickly. Used in clinical veterinary surgery, animals are prone to incomplete analgesia, muscle relaxation adverse, visceral issues such as stretch reflex. Xylidinothiazoline has been widely used in large animals and wildlife as chemical anesthetic drug. But ruminants have been prone to deep anesthesia, longer recovery time, bulging swollen rumen, stomach contents reflux and so on.Acupuncture anesthesia can reduce the side effects of narcotic drugs and the analgesic effect of drugs available. This experiment aimed at understanding anesthetic effect of the electro-acupuncture combined with xylidinothiazoline in goats and its impact on physiological and biochemical indicators, so that the basis for its clinical application was provided. Thirty healthy adult hybrid goats, age in the 1-1.5 years old, weighing 17-20kg, were randomly divided into 5 groups (n = 6): 0.5mg/kg xylidinothiazoline intramuscular group, intramuscular 2mg/kg xylidinothiazoline group, electro-acupuncture group, electro-acupuncture combined with 0.5mg/kg xylidinothiazoline group and control group. At 0,5,25,45,65and 95min (pure drug group) or at 0,25,45,65,85,115 min (electro-acupuncture or electro-acupuncture group of compound drugs) , DL-ZⅡDC sensors electrotherapy machine was used to determined pain threshold using potassium iontophoresis. PM-9000 Portable Multi-Parameter Monitor was used to determine the respiratory rate, heart rate and body temperature. The femoral artery pressure of goats was determined by electronic sphygmomanometer. Before the experiment and after the experiment 1.5, 24, 48, 96 and 168h goat blood collection, RT-1904C Semi-automatic biochemical analyzer was used to determined the serum alanine aminotransferase activity, alkaline phosphatase activity, urea concentration, creatinine concentration, aspartate aminotransferase activity, blood sugar levels and GC-911γ-counter radioimmunoassay was used to determined the cortisol levels. Veterinary pocH-100iV Diff automated hematology analyzer was used to analyze the peripheral blood erythrocyte number, leukocyte number and the ratio of neutrophil and Lymphocyte.In this experiment, the goats' pain threshold of electro-acupuncture combined with 0.5mg/kg xylidinothiazoline group and 2mg/kg xylidinothiazoline group had no significant difference, and that the analgesic effect of the former and the latter was considerable. Electro-acupuncture combined with 0.5mg/kg xylidinothiazoline group goat respiratory rate compared with the control group, there were no significant differences, and the respiratory rate was significantly lower than 2mg/kg xylidinothiazoline group. Electro-acupuncture had no significant impact on goat mean arterial pressure and heart rate. Conventional dose xylidinothiazoline (2mg/kg) can increase alanine aminotransferase, aspartate aminotransferase and serum alkaline phosphatase activity. In the experiment, xylidinothiazoline could significantly increased blood glucose, cortisol, serum urea and creatinine levels. Xylidinothiazoline had no significant impact on the number of red blood cells, but in peripheral blood the number of WBC, the ratio of lymphocyte increased after injected xylidinothiazoline.Electro-acupuncture combined with 0.5mg/kg xylidinothiazoline is indeed analgesic effect on goat, but the impact of goat physiological and biochemical indicators was no significant.