| The barley is one of most ancient crops in the world, which is fourth most important cereals crops after the wheat,the corn and the paddy rice. The barley mainly serves as the feed, the grain, the beer raw material for industry as well as pharmaceutical industry raw material and the health foods. In recent years, Gansu Province brewer's barley planter scale was expanded year by year, but the idioplasm resources day by day degenerated. For solving this problem, 26 new varieties were introduced from overseas to attempt with the local 9 varieties hybridizes and obtain the superiority variety by Gansu Province Institute of Agricultural Sciences Beer Raw material Research Institute. The SSR was used to analzize the genetic difference ofbarley and establish the optimum SSR-PCR reaction amplification system. The results was as following:1. Establish the optimum SSR-PCR reaction amplification system and procedures of barely. The method of orthogonal design was used with 4 levels of 4 factors, which were the template DNA, dNTPs, primers and Taq DNA polymerase concentration. Eventually, the optimal reaction amplification system and procedures was established.2. 32 preimers were screed from 49 SSR primers of 7 chromosomes in barely with using the SSR-PCR reaction amplification system, which can generate the reproducible fingerprinting profiles.3. A total of 200 alleles were detected among 32 loci, and 169 alleles were the polymorphic loci. The polymorphic proportion is 84.5%. Each primer amplificed 1-12 alleles, and average of 5.2 loci were produted by one primer. The PIC of EBmag0793 was maximum, which was 0.89. The PIC of EBmag0173 was miximum, which was 0.29. The results were indicated that GS among spices from to 0.05-0.6667.4. The SSR markers result of partial barely was analized by UPGMA. The genetic distance of 35 beer barely was at 0.26 levels. It was divided into four categories. The first broad categorie was consist of two subclasses. The first subclass was consist of two neatness. The first part is consist of KH.GYONGYOS,KH DAMA,KH LEDI,Jaxana,KH.SZOFI,9404-13-1-3,Z109M050M,PRESTIGE,Z090M066M. The second neatness is 8810,Ganpi No.2,Ganpi No.3,Ganpi No.4,Ganpi No.5,9404-13-3,Jubelete,Qinta,M-22 Z053N066N,MERIT,TRADITION,6B98-9339,H-84,Z014J081J, B1202,B1614. The second subclass was consist of 9413-6-3-3 and Jubilant. The second broad categories composed of Hungary's TABORA, Gansu local's 9416-4-1-3, USA Bush Company's Pasadena. The third category and the fifth category indnepedently composed of SCARLETT and Raxana.5.The majority of Ganpi Series varieties (lines) as 8810, Ganpi No.2, Ganpi No.3, Ganpi No.4, Ganpi No.5, 9404-13-3,9413-6-3-3,9404-13-1-3,the genetic basis was rather narrow. Only 9416-4-1-3 had relatively wide range with other Ganpi Series varieties (lines);Hungary's KH.GYONGYOS,KH DAMA,KH.SZOFI,KH LEDI,Jaxana had narrow of genetic basis with Ganpi Series varieties (lines) 9404-13-1-3 and USA Bush Company's Z109M050M,PRESTIGE,Z090M066M; 9413-6-3-3 had narrow genetic basis with Hungary's Jubilant. It will help improve the hybridity that the parents had relatively wide genetic basis when we select parents of hybrids. |
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