Compound In Veterinary Medicine "Like" Prevention And Treatment Of Diarrhea Diseases In Dogs

Dog diarrhea is a common clinical symptom, caused by many diseases and difficult to be prevented. According to the relevant information and clinical reports, diarrheas occurred in about 60 percent in the diseases in dog, which are harmful to the healthy of dogs. In order to find out a good therapy for treatment of dog diarrhea from Traditional Chinese veterinary medicine, the theory of "rule varies with the disease, different disease with the same method" was used as a guide to screen the preparation. Based on unique resources of Traditional Chinese veterinary medicine in northwest of China, the Chinese medicine compound preparation "Li ke" was developed. The following six experiments were carried out to meet the needs of developing this preparation further.(1) The screening and optimizion of the Chinese medicine compound preparation "Li ke" in clinic. "Li ke 1" revealed the best clinical efficacy through the prescription screening. The drug atced quickly with short course of treatment and good repeatability so it was chosen as final prescription named "Li ke".(2) The experimental dysentery efficacy of "Li ke". "Li ke" possessed good significant therapeutic effects in reduction of body temperature, slowing heart rate, promotion of blood white blood cells (WBC) and enhancing activity of lysozyme and increasing levels of INF-γ. This indicates that "Li ke" could regulate the immune functions so as to improve the ability of resistance to diseases.(3) The preliminary security evaluation of "Li ke". The toxicological test showed maximal tolerance dosage of "Li ke" was 160g/kg, therefore the security dosage of this medicine was equal to 213.3 times of clinical dosage indicating no or low toxicity in oral administration of "Li ke".(4) The antibacterial experiment of "Li ke" in vivo and in vitro. Sensitivities of avian Salmonella to "Li ke" were studied at concentration of 1 g/mL by measuring antibacterial circle and it's minimum inhibitory concentrations (MIC) against Salmonella of the drug. The antibacterial circle of the drug was 20.14 mm and MIC was 0.0625g/mL. The experiment revealed that "Li ke" had a strong assistance against infection of Salmonella, the survival rate of chicken was 70 percent. The antimicrobial experiment indicated that "Li ke" has a good inhibitory action to Salmonella infection.(5) Effect of "Li ke" on experimental diarrhea in mice and rabbit intestinal motility in vitro were investigateded. The result showed that "Li ke" could decrease the times of wet dung induced by castor oil, but had little effect on enteral diarrhea induced by Senna Leaf. "Li ke" inhibited the propulsion of small intestine and had an anti-diarrhea effect on diarrhea induced by the drugs in mice and rabbit.(6) The preliminary quality control of the "Li ke" was established. The content of forsythin was determined by HPLC. The results proved that determination of forsythiae satisfy the requirements of Chinese Pharmacopeia 2005 edition. Forsythin, protocatechualdehyde and kakkonein were idenficated by TLC. The control and the sample displayed the same spot on thin-layer at same position. The content of forsythin in "Li ke" was over 0.036mg/mL. The methods could be used for controlling the quality of "Li ke".The following conclusion can be drawn from the above tests that "Li ke" atced good repeatability, which can be able to provide a reliable basis for clinical application.