Experiment On The Prevention And Treatment Of Duck Infectious Serositis By Traditional Chinese Medicine

Duck infectious serositis(DIS)is a contact epidemic caused by Riemerella anatipestifer,which is a major hazard to birds such as geese,turkeys,ducklings.This disease is most susceptible to 2 to 8 days ducklings through the respiratory tract,skin and other routes.DIS is one of the most serious infectious diseases that endanger duck infustry,can cause high mortality rate of ducklings and growth retardation.The resistance of Riemerella anatipestifer to chemical drugs is becoming more and more serious.This paper carried out a series of experiments on the prevention and treatment of duck infectious serositis by traditional Chinese medicine.In this paper,bacteria were isolated and identified from diseased ducks suspected of infectious serositis in different areas,and carried out the pathogenic research.The bacteria were isolated from the duck brain of the diseased duck and then identified by identification culture,microscopic examination,biochemical test and PC R detection.As a result,six isolates of Riemerella anatipestifer were identified,the colony morphology,cell morphology,dyeing characteristics,biochemical reactio ns and the PCR sequencing results of all six isolates are in line with the basic characteristics of Riemerella anatipestifer.The isolates which were finally identified as Riemerella anatipestifer were amplified and cultured.The animal infection test was carried out and the pathogenicity of the isolates was investigated.The results showed that 5 out of 6 isolates have a high pathogenicity,the incidence of up to 80.00%.According to the C linical and Laboratory Standards Institute(CLSI),the minimum inhibitory concentration(MIC)of six isolates to a variety of drugs were measured using a micro-broth dilution method.The results showed that all isolates were highly sensitive to cephalosporins,amoxicillin and florfenicol,and the MIC values were less than 1.0 μg/mL.But had resistance to enrofloxacin,apramycin and neomycin,and the MIC values were greater than 8 μg/mL.The sensitivity of isolates from different regions to doxycycline and spectinomycin was different.Self-made traditional Chinese medicine,qingwenjiedu powder,jinyeqingwen powder,coptischinensis,pulsatilla and poplar flowers have a strong inhibitory effect on all isolates,the MIC values were below 32 mg/mL.In order to investigate the preventive effect of several traditional Chinese medic ine on duck infectious serositis,clinical trials was carried out.300 healthy muscovy duck lings were randomly divided into 5 groups: blank control group,positive control group and different drug prevention test groups.The test groups were given different test drugs before the artificial infection of bacteria were given.After dosing,all duckings of the experimental groups and the positive control group were injected with isolated Riemerella anatipestifer via intramuscular.The results showed that the positive control group,the morbidity and mortality rate were 98.33% and 80.00%,respectively.The morbidity of self-made Chinese medicine,qingwenjiedu powder and jinyeqingwen powder were 25.00%,28.33% and 35.00%,respectively,and the mortality rates were 13.33%,15.00% and 18.33%,respectively.A certain effect of three kinds of traditional medicine on the prevention of duck infectious serositis was revealed.In this paper,a systematic method for the isolation and identification of Riemerella Anatipestifer and the determination of drug sensitivity was provided.And the effects of various traditional Chinese medicine prescriptions on duck infectious serositis were investigated.This study could provide new ideas and methods for the clinical prevention and treatment of duck infectious serositis.