| In order to relieve livestock pollution and realize swine cleaner production,content of copper and manganese was lowered through replacing inorganic form with chelated form which was proven to have a higher bioavailability,without lowering growth rate.In this paper,effect of several proportions of Hydrolyzed feather meal Chelated copper and manganese on growth performance and immune function of weaned piglets were studied.Experiment 1:five little of 70 Duroc-lanndrae-Yorkshire cross weaned piglets about 10-12kg body weight were divided into five groups(A,B,C,D and Control).cheated copper and manganese in trial diets were replaced in corporation with control group,with 80%copper and 80%manganese in group A,60%copper and 80%manganese in group B,80%copper and 60%manganese in group C,60%copper and 60%manganese in group D.after 21-day,compared to control group,group D had similar average daily gain(ADG) to control group,while ADG of group A(0.66kg per day) raised by 8.2%,group B(0.63kg per day) by 3.3%,group C(0.63kg per day) by 3.3%,respectively. In case of feed/gain(F/G),group A<group B<group C<group D and control group.In case of diarrhea rate,result was similar to feed/gain and found group A best,group B next.Experiment 2:trial animal,group dividing,diet formula and experiment design were same to experiment 1.Ten piglets were randomly sampled and 5ml blood was drew from precaval vein,during 7:00-9:00a.m.at 7th and 21th day.blood coagulated and was centrifuged at 3000r/min for 1-2 minutes.separated serum was conserved at 4℃for late use of detecting activity of superoxide dismutase(SOD) and glutathione peroxidase(GSH-PX).Results suggested:group B achieved best SOD activity than the other groups,and either group B or group C had stable SOD and GSH-PX activity with a certain degree of rising inclination;when separately analyzed, GSH-PX activity rose as age,among which group C increased the most while group B,C,D all reached statistically significant level.Experiment 3:trial animal,group dividing,diet formula and blood drawing schedule were same to experiment 2.Content of serum immunoglobulin including IgA,IgG and IgM,was measured. Results indicated:content of IgA and IgM in group A significantly increased early at 7th day; content of IgG in all groups didn't significantly differ;totally,content of immunoglobulin in all groups had no significant change at 7th day and 21th day.Experiment 4:trial animal,group dividing,diet formula and blood drawing schedule were same to experiment 2.Swine Fever(SF) antibody blocking rate was compared between all groups. Results showed:group C had a significantly higher blocking rate,while group A,B and D better than control group,but not significantly.At 7th day,all trial groups were better than control group;at 21th day,except group D,group A,B and C,had significantly effect.Totally,group A(80%chelated copper and 80%chelated manganese) could serve as optimal scedule,although several replacing proportions of chelated copper and manganese had no agreeable effect on growth performance and immune function of weaned piglets. |