Effects Of Chelated Copper Proteinate And Chelated Zinc Proteinate On The Growing-Finishing Pigs

To study the effects of organic trace minerals on the antioxidant propertities of growing-finishing pigs, chelated copper proteinate and chelated zinc proteinate were supplemented in the diets of pigs in place of different proportions of trace minerals from inorganic form. The trace mineral content, activity of antioxidase and tissue damage of liver and spleen in growing-finishing pigs were determined. The paper included two trials.In experiment1, fourty-eight70-day-old pigs (Duroc×Large White X Landrace) were randomly allocated into3groups with4replicates,4pigs in each replicate. The pigs were group-fed basal diets supplemented with copper sulfate(control), chelated copper proteinate replacing of30and100percent of copper sulfate. The concentrations of trace minerals, activities of antioxidase and the malondialdehyde(MDA) content in liver and spleen and the activities of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST) of growing-finishing pigs were determined. The liver and spleen were obtained and then stained with HE to be observed with microscope to study their tissue morphology. The results show that the pigs fed diets with100%chelated copper proteinate had higher liver zinc and spleen manganese content than the control(P<0.05). An tendency of liver copper and spleen copper content to increase were observed in both chelated copper proteinate treatments(P>0.05); The pigs fed diets with100%chelated copper proteinate had lower MDA content in the live than the control(P<0.05). However, the activities of superoxide dismutas (SOD), catalase(CAT) and glutathione peroxidase(GSH-Px) activity in the liver were not affected among all the treatments(P>0.05). Both of the supplementation with100%chelated copper proteinate and30%chelated copper proteinate increased SOD activity and decreased MDA content in the spleen(P>0.05); No significant injury was observed among all treatments. The difference of ALT and AST among all treatments was not significant (P>0.05). These results indicate that the supplementation of chelated copper proteinate increases spleen antioxidase activity and decreases oxidative injury of live and spleen in growing-finishing pigs, but not obviously affects tissue morphology of liver and spleen. and the diet supplementation with chelated copper proteinate replacing of100percent of copper sulfate seems to have higher antioxidative response on growing-finishing pigs than that of30percent.In experiment2, fourty-eight70-day-old pigs (Duroc×Large White×Landrace) were randomly allocated into3groups with4replicates,4pigs in each replicate. The pigs were group-fed basal diets supplemented with zinc sulfate (control), chelated zinc proteinate replacing of30and100percent of zinc sulfate. The concentrations of trace minerals, activities of antioxidase and the malondialdehyde(MDA) content in liver and spleen and the activities of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST) of growing-finishing pigs were determined. The liver and spleen were obtained and then stained with HE to be observed with microscope to study their tissue morphology. The results show that the pigs fed diets with100%chelated zinc proteinate had higher liver zinc(P<0.01), liver copper(P<0.05) and spleen zinc(P<0.05) content than the control, and had higher liver zinc than pigs fed with30%chelated zinc proteinate (P<0.05); An increase of SOD activity in the liver and decrease MDA content in the spleen were observed in the treatment of100%chelated zinc proteinate (P<0.05). The pigs fed diets with chelated zinc proteinate tended to have higher liver GSH-Px, spleen SOD activities and lower liver MDA content than the control(P>0.05). The liver CAT activity was not affected among all the treatments(P<0.05). There were no significant difference in morphology of liver and spleen tissue and activities of ALT and AST among all the treatment(P>0.05). In conclusion, the supplementation of chelated zinc proteinate increases antioxidase activity and decreases oxidative injury of live and spleen in growing-finishing pigs, but not obviously affects tissue morphology of liver and spleen. And the diet supplementation with chelated zinc proteinate replacing of100percent of zinc sulfate seems to have higher antioxidative response on growing-finishing pigs than that of30percent.