Effect Of Culture Condition On The Growth Of Plantlet And Microtuberizing Capability Of Potato In Vitro

To probe into the effect of different potato varieties,light intensity and plantlet age on microtuberization,three aspects were investigated,and the results were as follows.1.Study on screening of culture medium for stringing plantletPotato(Solanum tuberosum L.) cv.Atlantic being as the trial material,the experiment through orthogonalty design researched the effects of sucrose and exogenous hormones(6-BA, B₉,and NAA) on growth of virus free plantlet in vitro.The results suggested that,for different traits of plantlet,the optimum combination was not same.As for the plantlet height,it was the best combination that MS was supplemented with 3%sucrose,0.01mg/L 6-BA,20 mg/L B₉ and 0.1 mg/L NAA.As for the plantlet stem diameter,it was the best combination that MS was supplemented with 12%sucrose,0.1mg/L 6-BA,20 mg/L B₉ and 0.02 mg/L NAA.As for the plantlet stem nodes,it was the best combination that MS was supplemented with 3% sucrose,0.1mg/L 6-BA,35 mg/L B₉ and 0.1 mg/L NAA.And as for the plantlet leaves,the best was the MS supplemented with 3%sucrose,0.1mg/L 6-BA,20 mg/L B₉ and 0.02 mg/L NAA.2.Study on effect of lighting intensity on plantlet in vitroPotato cv.Yushu-1,Atlantic,and Eshu-3 as materials were used in the experiment about lighting intensity,variety and gibberellin,and it was designed through crack design to investigate the effect of different lighting intensity and gibberellin concentration on plantlet growth of different potato varieties.The results indicated that the plantlet growing vigor of plantlets on the circumstances of 3000Lux lighting markedly outwent the plantlets under the weak lighting intensity. 3.Study on the inducement of lighting intensity for tuberization of potato in vitroPotato cv.Yushu-1,Atlantic,and Eshu-3 being as the trial materials,the culture medium for strong plantlet was substituted with that for inducement after the plantlets was cultured for a month.The trial was carried out with crack design concerned with lighting,plantlet quality and variety to study the difference of physiological traits and microtuberization at the different lighting intensity,variety and plantlet quality.The result suggested that under the circumstances of darkness and at the age of plantlet with 5-7 leaves,the microtuber diameter, tuberizing rate and microtuber yield were markedly higher than other transactions.