Research On Relieving Seed Dormancy And Study On Alternative Induction Of Explant Of Eascaphis Konlshli Hayata

The crust of Eascaphis konlshli Hayata making it waterproof and airtight renders the seed hard to recover from dormancy. This study showed that germination rates were different when seeds were dealt with different concentrations of acid or alkali. The germination rate was the highest when they were dealt with 50% H₂SO₄ for 10 min, whereas the rate was lower if the concentration of H₂SO₄ was 40% or 60%. The rate was zero when dealt with 20%, 40% or 60% alkali. The seed which was not stored in sand could not grow in the culture medium by tissue culture technique.The leaves of Eascaphis konlshli Hayata were used as explants in this research to discuss the effects of different hormonal conditions on the induction and culture of Eascaphis konlshli Hayata's callus with MS mediums. The results indicated that adding single hormone, such as 2, 4-D or NAA was disadvantageous for the induction of Eascaphis konlshli Hayata's callus. It also indicated that adding two kinds of different hormones at the same time could accelerate the induction of Euscaphis japonica's callus. The induced effect of 0.5 mg·L^-1 6-BA plus 1.5 mg·L^-1 NAA was optimal, and the induced rate of callus was up to 70%.