Studies On Callus Induction And Cell Suspension Culture Systems Of Perilla

The purpose of this study was to establish the system of tissue culture, cell suspension culture and root induction of Perilla.The seeds, cotyledons, and hypocotyls were treated as explants with alcohol, HgCl₂ and NaClO according to different concentrations and time. The result showed that the optimal sterilization method for the seeds was 75% alcohol for 20s and 0.1% HgCl₂ for 10min; as for hypocotyls and cotyledons, 75% alcohol for 20s and 0.1% HgCl₂ for 4min was better. Leaf sterilization experiment showed the optimal effect when treated with 75% alcohol for 30s and 0.1% HgCl₂ for 7min.The results showed that the callus induction rate was higher when the explant was treated with 2,4-D(0.1mg/L)for 3 days. Different varieties have different callus induction rates, Perilla frutescens var. auriculato-dentata is the optimal material for callus induction. Different explants also have different callus induction rates, and cotyledons and leaves are easier to produce calli. The optimal culture medium to induce calli is MS+NAA (1.0mg/L)+6-BA(2.5mg/L). This medium is also suitable for the callus subculture.Then the hard green calli were transferred into the liquid MS culture medium added with NAA (1.0mg/L) and 6-BA (2.5mg/L). The data of pH, sucrose concentration and electrical conductivity were detected every 4 days in order to get the growth curve.The leaves of Perilla frutescens var. auriculato-dentata were treated with 6-BA (0.4mg/L).Then the leaves differentiate into cluster of leaves and grow very fast, the roots grow 1 month later and a complete plant is developed 2 months later.