| Capparis spinosa L. is a very important plant in ecological and economical effect. However, the narrow distribution and the gradual decrease of resources restrictes its'application. This paper comprehensive describes the basic research from the actual requirement, which includes seed germination conditions, plant regeneration, callus induction and subculture, the physiological and biochemical characters on the cell suspension culture and the anatomy structure of vegetable organs. The purpose is to provide a scientific base for reasonable exploitation and utilization of Capparis spinosa L.. The main contents are as follow:1. This experiment tries to analyze the favorable conditions for the seed germination. The result shows that the Capparis spinosa L. seeds shown dormancy. The seed germination rate can be enhanced remarkable and germination time becomes shorter when it is treated with GA3 and KNO3. The seed coat and the planting time also have a great effect on the germination quality. And the optimum of planting time is spring.2. The effects of different plant growth regulators on germination, induction, multiplication and rooting of explants are studied in this paper. It is shown that the best medium for seed germination is MS+1.0 mg/L 6-BA +2% sucrose, the MS medium combined with 0.10 mg/L 6-BA and 0.02 mg/L NAA is suitable for shoot multiplication. And the multiplication rate is about 5-6 times of that without hormone. The MS medium supplemented with 1.00 mg/L 6-BA and 100 mg/L activated charcoal was best for rooting. The results show that the in vitro regenerated plantlets of Capparis spinosa L. have the characters of rapid growth and high propagation coefficient. At the time, the plantlets could normally root and survive after transplantation.3. The young leaf has better effect on induction of callus than that of stem and seed. And the suitable disinfection method is 70% alcohol 20 s with 0.1% HgCl2 8 min. The influence of different hormone concentration on induction and subculture of the callus is also discussed. And the main factor, which affects callus , is 6-BA. The results show that the appropriate culture medium for induction and multiplication were MS+1.0 mg/L 6-BA + 0.5 mg/L 2, 4-D and MS+1.5 mg/L6-BA+1.0 mg/L 2,4-D, respectively.4. The kinetic of Capparis spinosa L. cell growth and nutrient consumption in suspension culture is investigated. It is revealed that cell growth curve, which is about 30 days, is belong to"S"type. And the biomass multiplication rate is about 2.8 times. In the growth cycle, sucrose was ingested quickly, but there is a little of reducing sugar and soluble total sugar left in the end. Peroxidase isozymes changes during the cell growth course. And there is a positive correlation between sucrose concentration and POD enzyme activity level.5. Capparis spinosa L. is a perennial shrub plant, which is resistant to drought and hot. Anatomic structure of root, stem and leaf were studied by paraffin section method. The results indicate that the Capparis spinosa L. has obvious characteristics to adapt the drought environment. The leaf has up growth palisade tissue and epitrichoderm. And the hollowed stomas are distributed on lower and basal surface. The epidermis cell was tightly arranged. Furthermore, the vascular is developed and the periderm is obvious in the stem. All those results indicate the strong resistance to drought environment.
|
PDF Full Text Request