Effects Of Rancid Oil On The Flora In Intestinal Broiler

In this paper the effects of rancid oil added in diet for broiler on the flora of their alimentary canals were investigated to provide information of controlling secondary and complicated diseases and improving their feed conversion. With the ecological methodology of quantitative and qualitative analyses, normal (4) and rancid oil at different acid values (8 and 10) were utilized in the diet for the chicks in group 1 as control, group 2 as treatment 1 and 3 as treatment 2, in order to find out the oil-added effects on the physiological indexes of the flora, i.e. enterococcus, escherichia, bifidobacteria, lactobacilli, fusobacteria, Eubacteria, Bacteroides, which perch in such positions of alimentary tract as crop, gastritis, duodenum jejunum, ileum, cecum and rectum.When the chicks were 15 day's old, echerichia in control (lowest) showed no difference from in treatment 1 but significantly lower than the treatment 2(p<0.05) . At gastritis, jejuna, ileum and cecum, with the enterococci in the crop, cecum and rectum, the two treatments did not differ but were all lower than the control (p<0.05), while the control and treatment 1 at the same level were higher (p<0.05) than the rest group in gastritis, jejuna and ileum. Bifidobacteria in jejuna had no diference between the 2 treatments but lower than the control (p<0.05) , and in rectum, control and treament 1 show no diference but higher (p<0.05) than the treatment 2. With lactobacilli, no diference were found between control and treament 1 in ileum and rectum, and the rest treatment had the lowest (p<0.05) value; menwhile, in cecum, the 2 treatments were lower (p<0.05 ) than the control. In the crop, fusobacteria of treatment 1 showed no diference with control and these two groups were wery lower (p<0.01) than the treatment 2; at the level of ileum, the germ's significant diference (p<0.05) only exist with the treatment 2, and there was higher values(p<0.01) in the two treatments than in control at the positions of duodenum, cecum and rectum. As for the bacteroides and eubacteria, no significant change was observed among the 3 groups. The trials suggested that balances of the micro eco-system in the alimentary canal were chalenged because of the rancid oil added, even worse in the treatment 2 and abnormal in treatment 1 to some extent.On 20th day, the two treatments (no diference) of echerichia were higher(p<0.01) significantly than the control in crop, cecum and rectum, in gastritis the germ were only higher (p<0.05) in treatment 2, and in jejulum also only significant higher value (p<0.01) was found in treatment 2. Bifidobacteria in rectum showed significant high level (p<0.01) in control and treatment 1compared to the treatment 2. Lactobacilli of the treatments with no diferencewere significantly lower (p<0.01) than control in crop, gastritis and ileum.Bacteroides and eubacteria had no diference between the treatments but higher(p<0.01) than control. As for the fusobacterium, very significant high values(p<0.01) were found in the two treatments compared to the control in crop,gastritis, ileum and cecum. It was suggested that imbalence of the microeco-system in digestive system led the disorder of the flora.Until 25 days later, general speaking, significantly more (p<0.01) scherichia and fusobacteria of the treatments were observed than of the control all in the alimentary tract while no change existed between the treatments. And there were significantly more (p<0.01) enterococcus, bifidobacteria, lactobacilli, eubacteria and bacteroides in the control than in the treatments at all positions of the tract, while treatment 2 was lower (p<0.01) than treatment 1. It indicated that the order of flora in treatment 2 was more severely injured than in treatment 1.The trials demonstrated that the colonization resistance (B/E ) were weaker of the two treatments of chicks than of the control, and the treatment 2 was even worse, so the pathogenic germs were easy to invade and set in and followed by poor disease resistance, high morbidity, retarded growth and poor feed conversion consequently.