| Intestinal micro flora have an important role in mammal's health and disease .As the industry and agriculture's development, a small quantity of antibiotics residue in the animal products through the drinking and food . these residue maybe enter the human body through the drink and food and change the ecological balance of intestinal micro flora .so ,some international organizations such as Food and Agriculture Organization/ world health organization give some guidelines and suggestion about human intestinal micro flora safety evaluations, and formulate some antibiotics ADI.Yet, our nation has not formulate the procedures of human intestinal micro flora's safety evaluations on animal antibiotic residue and the ADI in our country enacted according the standard of JECFA. Whether the ADI safe or harmful and it is an urgent thing that how to set the safety evaluation on antibiotic residues.The common detection method usually established on the plat count, because of the limitation of the intestinal micro flora culture method, a great quantity of researchers begin to use the molecule fingerprint technology to analysis the intestinal micro flora . the DGGE technology has the quality of direct and quick ,fit to analyses the complicated intestinal micro flora.This research choose the Enrofloxacin which widely used in veterinary surgeon clinical, applied the DGGE technology and plat count to analyze the residue's influence on the quantity , diversity and colonization resistance (CR)in SPF rat's intestinal micro flora. the experiment composed by two parts: The first groupThe purpose: applied the DGGE technology and plat count to analyze the residuce's influence on the quantity, diversity and CR in SPF rat's intestinal micro flora.The method: breed male SPF rank KM rat for 7d , take orally Enrofloxacin water solution for 35d, and then natural recovered for 21d. use the DGGE technology and plat count to analyze the quantity and diversity changes in SPF rat's intestinal microflora in all the experiment: period.The result :the analysis of the DGGE indicates that in the four density Enrofloxacin treatment groups. The rat intestinal microflora which treated by 0.03mg/L Enrofloxacin,A diversity consistent with the rat which did not deliver drug ,the result of SPSS13.0 has no significant difference ; when treated with 0.3mg/L, SPSS13.0 indicates that the drug make effect with the evenness (P<0.05) ; and when treated with 3mg/L,30mg/L,the diversity in the rat intestinal microflora has significant difference with the blank rat (P<0.05) .Plat count result displayed the aerobe in the high dose group (P<0.01 ),middle dose group (P<0.05) and the quantity of enteric bacilli in high dose group (P<0.01) reduced after the treatment. The aerobe and anaerobe in high dose group and middle dose group's drag fast rate increased (P<0.01) .The second groupThe purpose: use the plat count to analyze what effect mar be caused by different Enrofloxacin residue in SPF rat micro flora.The method: breed male SPF rank KM rat for 7d , take orally Enrofloxacin water solution for 35d, take orally larvae at the 11d,12d for twice , and then natural recovered for 21d. use the plat count to analyze the CR in SPF rat's intestinal microflora in all the experiment period.The result: SPSS13.0 results indicates that the high dose and middle dose group's CR (clearing ability to Blastomyces albicans) weaker (P<0.05) .In the research , PCR-DGGE and count plate are combined to evaluate the safety of the microbe antibacterial agent residuce and we also studied the quality and diversity of intestinal tract microbial population imposed by different doses of Enrofloxacin. The result proved that PCR-DGGE is more sensitive than plate count, and it is a good technology which can be used in the antibacterial agent microorganism safety evaluation ; the research also indicated that the ADI in our county may influenced the diversity of human intestinal tract flora.
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